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Diluent for normal temperature preservation of sperm of lepus yarkandensis under artificial domestication conditions and preparation

A technology of artificial domestication and normal temperature preservation, which is applied to the preservation, application, and animal husbandry of human or animal bodies. It can solve problems such as unclearness, unsatisfactory semen preservation effect, and difficult application, etc., to achieve weakening Oxidative stress, maintaining sperm motility and plasma membrane integrity, and improving the chance of conception

Inactive Publication Date: 2018-05-29
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In recent years, the semen storage diluent prepared for rabbits at room temperature (Li Shidong rabbit semen storage diluent at room temperature and its preparation method publication number CN102318596A) and donkey semen cryopreservation method (Liu Xinfeng a donkey semen cryopreservation method publication number CN107183013A) and the effect of artificial insemination have been carried out some researches, and the method for the diluent prescription of the normal temperature preservation diluent formula of these research designs and donkey semen is not easy to be applied in the Tarim rabbit artificial domestication and breeding, and the semen preservation effect has not been obtained. satisfactory effect
However, whether the diluent formula for normal temperature storage of rabbit semen is suitable for the preservation of Tarim rabbit semen is not clear

Method used

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  • Diluent for normal temperature preservation of sperm of lepus yarkandensis under artificial domestication conditions and preparation
  • Diluent for normal temperature preservation of sperm of lepus yarkandensis under artificial domestication conditions and preparation
  • Diluent for normal temperature preservation of sperm of lepus yarkandensis under artificial domestication conditions and preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The diluent of Tarim rabbit semen stored at room temperature under artificial domestication conditions was prepared by adding 30g of Tris, 20g of citric acid, 1g of glucose, 10ml of egg yolk, 1 million IU of penicillin, 1 million IU of streptomycin, and adding double distilled water to make up to 1L.

[0039] The preparation steps are: (1) Measure and weigh 30g of Tris, 20g of citric acid, and 1g of glucose, add double distillation to 0.5L, and shake well; (2) Dissolve the solution obtained in step (1) in a 30°C water bath for 20 After 10 minutes, shake well, after cooling to room temperature, add 10ml of egg yolk, 1 million IU of penicillin, 1 million IU of streptomycin, stir evenly, add double distilled water to make the volume to 1L, shake well; The pH is 6.8.

[0040] Slowly add the prepared dilution to the male rabbit semen, slowly add the dilution in portions and shake gently, and dilute to 3-5 times according to the quality of the semen, and store the diluted sem...

Embodiment 2

[0044] Semen preservation, method of use and test based on the first embodiment.

[0045] Tarim rabbit semen stored at room temperature under artificial domestication conditions is prepared by adding 34.5g of Tris, 24.5g of citric acid, 5g of glucose, 20ml of egg yolk, 1.4 million IU of penicillin, and 1.4 million IU of streptomycin, and adding double distilled water to make up to 1L. become.

[0046] The preparation steps are: (1) Measure and weigh 34.5g of Tris, 24.5g of citric acid, and 5g of glucose, add double distillation to 0.5L, and shake well; (2) Put the solution obtained in step (1) in a 30°C water bath After dissolving in medium for 20 minutes, shake well, after cooling to room temperature, add egg yolk 20ml, penicillin 1.4 million IU, streptomycin 1.4 million IU, stir evenly, add double distilled water to make up to 1L, shake well; you can get the dilution, The pH of the diluent was 7.0.

[0047] The results show that the Tarim rabbit semen storage diluent at ro...

Embodiment 3

[0049] Semen preservation, method of use and test based on the first embodiment.

[0050] Tarim rabbit semen stored at room temperature under artificial domestication conditions is prepared by adding 35.5g of Tris, 25.5g of citric acid, 6g of glucose, 25mL of egg yolk, 1.5 million IU of penicillin, and 1.5 million IU of streptomycin, adding double distilled water to make up to 1L. into, the pH value of the diluent is 7.2.

[0051] The preparation steps are: (1) Measure and weigh 35.5g of Tris, 25.5g of citric acid, and 6g of glucose, add double distillation to 0.5L, and shake well; After dissolving in the medium for 20 minutes, shake well, after cooling to room temperature, add egg yolk 25ml, penicillin 1.5 million IU, streptomycin 1.5 million IU, stir evenly, add double distilled water to make the volume to 1L, shake well; you can get the dilution, The pH of the diluent was 7.2.

[0052] The results show that the Tarim rabbit semen storage diluent at room temperature under ...

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Abstract

The invention relates to a diluent for normal temperature preservation of sperm of lepus yarkandensis under artificial domestication conditions and preparation, and belongs to the technical field of wild animal reproduction. The diluent provided by the invention is prepared from 30g-40g of Tris, 20g-30g of citric acid, 1g-10g of glucose, 10ml-50ml of yolk, 1,000,000IU-2,000,000IU of penicillin, 1,000,000IU-2,000,000IU of streptomycin and the balance of double distilled water which is added until the constant volume is 1L. The pH value of the diluent is 6.8-7.4. The diluent provided by the invention can effectively prolong the normal temperature preservation time of the sperm of the lepus yarkandensis by 32 hours and can obviously improve the pregnancy probability and the litter size of female lepus yarkandensis under artificial domestication conditions, wherein the pregnancy rate is 60% or more, and the litter size is 2 or more.

Description

technical field [0001] The present invention relates to the technical field of wild animal reproduction, in particular, the present invention relates to the technical field of a diluent for storing Tarim rabbit semen at room temperature under artificial domestication conditions and its preparation. Background technique [0002] The Tarim Basin is the largest inland basin in the world. It is located in the southern part of Xinjiang Uygur Autonomous Region, from the eastern foot of the Pamirs in the west to the Lop Nur depression in the east, to the southern foot of the Tianshan Mountains in the north, and to the northern foot of the Kunlun Mountains in the south. The Tarim Basin is a typical inland closed area surrounded by the Tianshan Mountains, the Kunlun Mountains, the Altun Mountains and the Pamirs. Because the surrounding mountains block the warm and humid air flow from the Atlantic Ocean, the entire basin presents a typical continental climate. The temperature in the ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 库尔班·吐拉克王重生木沙·托合提陈海燕
Owner XINJIANG AGRI UNIV
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