Preparation method of scar removal drug for external application
A medicament, a technology for removing scars, applied in the directions of pharmaceutical formulations, preparations for skin care, medical preparations with non-active ingredients, etc. Generation, promotion of fibroblast and collagen fiber production, free radical scavenging effect
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[0026] A preparation method for externally applied medicament for removing scars, comprising the steps of:
[0027] (1) Add aloe, angelica dahurica, Chinese yam and baicalin into distilled water according to the mass ratio of 7:5:4:5:100, soak for 3~5h, mash, filter, take the filtrate A, and pour the filtrate into the filtrate according to the mass ratio of 1:10 Add ethanol solution to A, shake for 2~3 days, filter, take the filtrate B, and rotary evaporate to obtain the ethanol-free mixture, concentrate to obtain the concentrated mixture, add distilled water to dissolve the concentrated mixture at a mass ratio of 1:20, and vacuum dry to obtain the mixed Chinese medicine powder, spare;
[0028] (2) Add the pretreated extraction materials to Tris-HCl buffer at a mass ratio of 1:4, homogenize in an ice bath at -2°C, extract at 4~5°C for 24~36h, 4°C, 10000r / min Centrifuge for 30 min, take the supernatant, and get the extract of Chiziai earthworm, and set aside;
[0029] (3) Tak...
example 1
[0032] Add aloe, angelica dahurica, Chinese yam and baicalin into distilled water at a mass ratio of 7:5:4:5:100, soak for 3 hours, mash, filter, take filtrate A, add ethanol solution to filtrate A at a mass ratio of 1:10 , vibrate for 2 days, filter, take the filtrate B, and rotary evaporate to obtain the ethanol-free mixture, concentrate to obtain the concentrated mixture, add distilled water to dissolve the concentrated mixture at a mass ratio of 1:20, and vacuum dry to obtain mixed Chinese medicine powder. Apply the mixed Chinese medicine powder on the hands of the subjects twice a day to test the melanin-inhibiting and antioxidant properties of the mixed Chinese medicine powder. Skin melanin content MI value: Based on the principle of spectral absorption (RGB), the content of melanin in the skin is determined by measuring the amount of reflection of light of a specific wavelength on human skin. The measurement range of the instrument is 0-999, the higher the measurement v...
example 2
[0037] Pretreatment of bee pollen: Purchase unbroken bee pollen, pass through an 80-mesh sieve to remove other bee pollen and impurities, dry at 70°C, and grind to obtain pretreated bee pollen.
[0038] Eisenia chinensis: purchased from an earthworm farm.
[0039] Pretreatment of extracted raw materials: After the live worms are washed with deionized water, the filter paper absorbs the surface moisture of the live worms, and the concentration is 0.01mol / L Tris at a pH of 7.0 in an ice bath at -4°C -In HCl buffer solution, use a sterile scalpel to cut off the 1 / 3 of the worm near the tail, mark it, take the first 2 / 3 of the worm and put it back in the soil to grow freely for 2 days, then take the worm and cut it off The newly grown body parts in the body must be pre-processed to extract raw materials.
[0040] Pretreatment of Eucheuma: Take Eucheuma, wash repeatedly with tap water twice to remove sediment and debris on its surface, then repeatedly rinse once with distilled wat...
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