A method to promote in vitro expansion of mouse retinal precursor cells by inhibiting microRNAs

Abstract

The invention discloses a method for promoting the in vitro expansion of mouse retinal precursor cells by inhibiting microribonucleic acid. MicroRNA miR-449a-5p is a non-coding single-stranded RNA molecule of 22 nucleotides in length, and its coding DNA sequence is located on mouse chromosome 13. Retinal precursor cells are a class of cells with self-renewal ability and the potential to differentiate into retinal neurons and glial cells. By synthesizing the antisense strand of miR-449a-5p and using the principle of base pairing to interfere with the level of endogenous miR-449a-5p, it can effectively promote the expansion of retinal precursor cells in vitro. Since retinal precursor cells are the developmental source of photoreceptor cells in the mature neural retina, their efficient in vitro expansion method could facilitate cell therapy based on stem / precursor cell transplantation.

Description

Technical field:

[0001] The invention belongs to the field of stem cell application, and in particular relates to a miR-449a-5p inhibitor sequence that can promote the expansion of mouse retinal precursor cells in vitro. Retinal precursor cells have important application potential in the damage repair of retinal diseases, so this expansion method can be applied to the treatment research of various retinal damage and degeneration models. In addition, because the sequence of mouse and human miR-449a is the same, this method is expected to be applied to the in vitro expansion of human retinal precursor cells. Background technique:

[0002] microRNA is a kind of non-coding single-stranded RNA molecule encoded by endogenous genes with a length of about 22 nucleotides. Participate in post-transcriptional modification and regulate the expression of target mRNA. It can down-regulate the expression level of protein by promoting the degradation of mRNA or preventing the translation ...

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