Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of freeze-dried MPO (myeloperoxidase) calibrator and freeze-drying protection solution

A technology of myeloperoxidase and freeze-dried protective solution, which is applied in the field of biochemical reagents, can solve the problems of poor resolubility of freeze-dried products, inconvenient transportation, short storage time of myeloperoxidase calibrator, etc.

Active Publication Date: 2018-06-01
GUANGZHOU JINDE BIOTECH
View PDF7 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a preparation method of myeloperoxidase lyophilized calibrator and a lyophilized protective solution to solve the problem of short storage time of myeloperoxidase calibrator and transportation problems. Inconvenient; problem of poor reconstitution of lyophilized products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of freeze-dried MPO (myeloperoxidase) calibrator and freeze-drying protection solution
  • Preparation method of freeze-dried MPO (myeloperoxidase) calibrator and freeze-drying protection solution
  • Preparation method of freeze-dried MPO (myeloperoxidase) calibrator and freeze-drying protection solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] As an embodiment of the freeze-dried protection solution of the myeloperoxidase freeze-dried calibration product described in the present invention, the freeze-dried protection solution of the myeloperoxidase freeze-dried calibration product of the present embodiment includes the following concentration and weight percentage: Components:

[0043] Phosphate buffer 20mM (pH 7.4), bovine serum albumin 1.0wt%, mannitol 1.0wt%, protein stabilizer 0.5wt%, polyethylene glycol 20000 0.5wt%, edetate disodium 0.2wt% , Tartrazine 0.005wt% and KY1000.1wt%.

Embodiment 2

[0045] As an embodiment of the freeze-dried protection solution of the myeloperoxidase freeze-dried calibration product described in the present invention, the freeze-dried protection solution of the myeloperoxidase freeze-dried calibration product of the present embodiment includes the following concentration and weight percentage: Components:

[0046] MOPS buffer 10mM (pH is 7.2), bovine serum albumin 0.5wt%, mannitol 5wt%, protein stabilizer 1wt%, polyethylene glycol 10000 0.2wt%, edetate disodium 0.4wt%, bright Blue 0.002wt%, ProClin3000.005wt% and KY100 0.005wt%

Embodiment 3

[0048] As an embodiment of the freeze-dried protection solution of the myeloperoxidase freeze-dried calibration product described in the present invention, the freeze-dried protection solution of the myeloperoxidase freeze-dried calibration product of the present embodiment includes the following concentration and weight percentage: Components: Tris-HCl buffer 50mM (pH 7.6), bovine serum albumin 3wt%, mannitol 0.5wt%, protein stabilizer 0.1wt%, polyethylene glycol 30000 2wt%, edetate disodium 0.05 wt%, β-carotene 0.01 wt%, ProClin300 0.1 wt%, and KY100 0.1 wt%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a freeze-dried MPO (myeloperoxidase) calibrator and a freeze-drying protection solution. The freeze-drying protection solution is prepared from bovine serum albumin, mannitol, a protein stabilizing agent, polyethylene glycol and disodium ethylenediamine tetraacetate. The MPO calibrator can be shrink-resistant after being free-dried with the freeze-drying protection solution, can keep the shape before freeze-drying, does not stick to a bottle and has better toughness; freeze-dried powder cannot be stuck on a bottle plug; a freeze-dried product canbe completely dissolved, and the solution is clear and is free of blocks and foam; activity keeps unchanged basically before and after freeze-drying; stability of the MPO calibrator can be improved remarkably, transportation of a freeze-dried antigen is facilitated, retention time of the MPO calibrator is prolonged, and retention time of an MPO detection kit is prolonged.

Description

technical field [0001] The invention relates to the technical field of biochemical reagents, in particular to a method for preparing a myeloperoxidase freeze-dried calibrator and a freeze-dried protective solution. Background technique [0002] At present, most of the calibrators in domestic myeloperoxidase (MPO) detection kits are liquid, and the MPO antigen is prone to denaturation in the liquid state. Although adding some protective agents (such as some proteins, sugars, and antioxidants, etc.) can prolong the MPO The storage time of the antigen in the liquid state, but the shelf life of the MPO antigen is generally 6-12 months when placed at 2-8°C in the presence of a protective agent. [0003] Some MPO calibrators at home and abroad also exist in freeze-dried form. After lyophilization, the MPO antigen has a long stable time and high accuracy, but the price is high; some kits use a high-concentration MPO lyophilized The stability of the dissolved high-concentration MPO...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/573G01N1/28
CPCG01N1/28G01N33/573G01N2001/2893
Inventor 段朝晖罗宁李民友蓝媛刘忠贵
Owner GUANGZHOU JINDE BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com