Super-resolution fluorescent lifetime imaging method and device based on parallel detection

Abstract

The invention discloses a super-resolution fluorescent lifetime imaging device based on parallel detection. The device comprises a light source, wherein exciting light sent by the light source is focused to a sample through a microobjective and signal light sent by the sample is collected, wherein a detection system receiving the signal light comprises a plurality of fiber bundles, a detector array and a time related single photon counter array; the plurality of fiber bundles receive the signal light at the same time; the detector array is provided with a plurality of detectors separately connected to fibers to obtain corresponding light intensity signals; and the time related single photon counter array is provided with time related single photon counters which are separately connected tothe detectors and are synchronized with the pulse of the light source for calculating the fluorescent lifetime and achieving super-resolution fluorescent lifetime imaging. The invention also discloses a super-resolution fluorescent lifetime imaging method based on parallel detection. By means of parallel APD and parallel TCSPC, the imaging resolution is enhanced, and the lifetime imaging speed isalso remarkably improved.

Description

technical field [0001] The invention belongs to the field of super-resolution microscopic fluorescence lifetime imaging, in particular to a super-resolution fluorescence lifetime imaging method and device based on parallel detection. Background technique [0002] The interaction between light and matter is the most basic interaction process in nature, especially the interaction between a single atom and a single photon is the most common element to realize the interaction between light and matter. In this field, super-resolution fluorescence microscopy has always been the focus of biomedical research. Through the special labeling of biomolecules, fluorescence optical microscopy plays an important role in observing subcellular structures. [0003] Fluorescence information has four basic physical dimensions, including intensity information, wavelength information (absorption spectrum and emission spectrum), lifetime information and polarization information. The current super-...

AI Technical Summary

Problems solved by technology

Lifetime measurement based on confocal system and TCSPC counting device is a kind of time-domain lifetime imaging method, which can provide better lifetime imaging resolution and can flexibly measure long and short-lived samples with various dynamic ranges, but this Due to the influence of the detector and counter dead time, the detection and counting efficiency of photons is low, so the imaging speed is very slow, which is also the biggest disadvantage of this method

Images