Preparation method and application of chicken manure protecting agent in production of chicken coccidiosis vaccine

A technology of vaccine production and chicken manure, applied in application, preservation of human or animal bodies, animal husbandry, etc., can solve the problems of manpower, material resources, time waste, and gradual decline of oocyst vitality, so as to prevent corruption and prolong storage Time, good effect of protection effect

Inactive Publication Date: 2018-06-08
TIANJIN HLINTE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the production of coccidiosis vaccines, it is often necessary to collect chicken manure for 5 days or even 10 days to extract oocysts from it. During the storage of fresh manur...

Method used

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  • Preparation method and application of chicken manure protecting agent in production of chicken coccidiosis vaccine
  • Preparation method and application of chicken manure protecting agent in production of chicken coccidiosis vaccine
  • Preparation method and application of chicken manure protecting agent in production of chicken coccidiosis vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A chicken coccidian oocyst protection solution, the components and weight of the protection solution are respectively:

[0027] Fructose 8kg, polyvinylpyrrolidone (10%) 4kg, sodium azide 0.06kg, sulfuric acid (98%) 0.04kg; sterilized water 87.9kg.

[0028] A preparation method and application thereof of chicken manure protective agent in the production of chicken coccidiosis vaccine, the steps of the preparation method are:

[0029] (1) Weigh 43.95kg of sterilized water and 8kg of fructose, first put the used sterilized water into the liquid mixing tank, then slowly add all the fructose into the liquid mixing tank, and stir while adding until the fructose is completely dissolved.

[0030] (2) Weigh polyvinylpyrrolidone, slowly add it into the liquid mixing tank of the above step (1), and stir while adding until it is evenly mixed.

[0031] (3) Take sodium azide, dissolve it with 8.79kg of sterilized water, slowly add the gained solution in the liquid mixing tank, and s...

Embodiment 2

[0035] A chicken coccidian oocyst protection solution, the components and weight of the protection solution are respectively:

[0036] Fructose 12kg, polyvinylpyrrolidone (10%) 5kg, sodium azide 0.09kg, sulfuric acid (98%) 0.06kg; sterilized water 82.85kg. The steps of its preparation method are the same as in Example 1.

Embodiment 3

[0038] A chicken coccidian oocyst protection solution, the components and weight of the protection solution are respectively:

[0039] Fructose 6kg, polyvinylpyrrolidone (10%) 3kg, sodium azide 0.04kg, sulfuric acid (98%) 0.03kg; sterilized water 90.93kg. The steps of its preparation method are the same as in Example 1.

[0040] Clinical application

[0041] Test 1, oocyst sporulation rate comparison test:

[0042] Inoculate 50 30-day-old AA broilers with the Eimeria tenella vaccine strain, 10,000 sporulated oocysts per bird. Collect 30kg of feces on the 7th day after inoculation, mix the 30kg of feces and divide them into 3 parts.

[0043] For the first group of 10kg feces, oocysts were extracted on the same day, sporulated on the same day, and marked as No. 1 oocyst after sporulation was completed;

[0044] The second group of 10kg feces, preserved for 4 days with the protective agent of the present invention, extracted oocysts and sporulated, marked as No. 2 oocysts aft...

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PUM

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Abstract

The invention relates to a preparation method and an application of a chicken manure protecting agent in production of a chicken coccidiosis vaccine. A composition comprises the following ingredientsin parts by weight: 1-10 parts of fructose, 1-10 parts of polyvinyl pyrrolidone (10%), 0.01-0.1 parts of sodium azide, 0.01-0.05 parts of sulfuric acid (98%) and 50-98 parts of sterile water. A formula composition of the agent is scientific and reasonable; a technology is simple; and the agent is convenient to use and very low in cost and has a better protecting effect on coccidian oocysts in manure. The protecting agent employing the ingredients such as the fructose serves as the protecting agent of the chicken manure in the production of the chicken coccidiosis vaccine can effectively keep activity of the coccidian oocysts in the chicken manure, prolongs preservation time of the manure, facilitates scale production and improves production efficiency. The manure preserved by the protecting agent can be preserved for 4 days at a room temperature, and the activity of the oocysts has no change as compared with that of oocysts in the fresh manure.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, in particular to a preparation method and application of a chicken manure protective agent in chicken coccidiosis vaccine production. Background technique [0002] With the continuous improvement of people's living standards, food safety issues are getting more and more attention. As the main source of animal protein, chicken and eggs have been repeatedly pushed to the forefront by public opinion, and the reason is the problem of drug residues. Chicken coccidiosis is a common disease that every chicken practitioner cannot avoid. The prevention and treatment of this disease mainly depends on the addition of chemical drugs such as salinomycin to the feed to prevent it. When it occurs, it is treated with antibiotics such as sulfonamides. , These chemical drugs not only pollute the environment during production and use, but also cause drug residues in chicken. As developed countries su...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 孙建李迎梅安同伟
Owner TIANJIN HLINTE BIOTECH CO LTD
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