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Application of dihydromyricetin in preparation of drug for treating kidney cancer

A kind of technology of dihydromyricetin and therapeutic drug, applied in the field of medicine

Inactive Publication Date: 2018-06-08
国药集团健康产业研究院有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of dihydromyricetin in the treatment of kidney cancer has not been reported yet.

Method used

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  • Application of dihydromyricetin in preparation of drug for treating kidney cancer
  • Application of dihydromyricetin in preparation of drug for treating kidney cancer
  • Application of dihydromyricetin in preparation of drug for treating kidney cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the preparation of dihydromyricetin in Hovenia dulcis fruit

[0026] Dried Hovenia dulcis 1.7kg, crushed through a 100-mesh sieve, added 1.7L of 60% ethanol to reflux extraction at 90°C for 2 hours, filtered with suction, collected extract 1 and filter residue 1; obtained filter residue 1 and 1.7L Mix 60% ethanol, reflux extraction at 90°C for 2 hours, suction filter, recover extract 2 and filter residue 2, mix filter residue 2 with 1.7L 60% ethanol, reflux extraction at 80°C for 2 hours, pump Filtrate and recover to obtain extract 3 and filter residue 3; combine extract 1, extract 2, and extract 3, and concentrate the extract under reduced pressure at 90 ° C to obtain 170.6 g of ethanol crude extract dry powder; take 150 g of Hovenia dulcis fruit crude extract Suspend in 1.5L of distilled water, extract with 4.5L of ethyl acetate, centrifuge the emulsified layer at 5000r / min, 4°C for 10min to separate into aqueous phase 1 and organic phase 1, pour out org...

Embodiment 2

[0028] Embodiment two, the preparation of dihydromyricetin in rattan tea

[0029] Grind 1700g rattan tea, pass through a 100-mesh sieve, add 1.7L 60% ethanol to reflux extraction at 90°C for 2 hours, filter with suction, collect extract 1 and filter residue 1; filter residue 1 obtained with 1.7L 60% Mix ethanol, reflux extraction at 90°C for 2 hours, suction filtration, and recover extract 2 and filter residue 2, mix filter residue 2 with 1.7L 60% ethanol, reflux extraction at 80°C for 2 hours, suction filtration, Recover extract 3 and filter residue 3; combine extract 1, extract 2, extract 3, and concentrate the extract under reduced pressure at 90°C to obtain 153.2 g of ethanol crude extract dry powder; take 150 g of vine tea crude extract and suspend in Extract 1.5L of distilled water with 4.5L of ethyl acetate, centrifuge the emulsified layer at 5000r / min, 4°C for 10min to separate into aqueous phase 1 and organic phase 1, pour out organic phase 1; 4.5L of ethyl acetate w...

Embodiment 3

[0030] Embodiment three, dihydromyricetin and Zn 2+ 、Cu 2+ 、Ni 2+ Reactive combination to form a complex

[0031] Accurately weigh three parts of 60mg (0.2mmol) of the dihydromyricetin composition obtained in Example 1 and add respectively in three 50ml round bottom flasks, add 20ml of absolute ethanol each, stir fully, add sodium acetate to adjust the pH to 7.5, each Add the same amount of zinc acetate, copper acetate, and nickel acetate, heat the flask in a 60°C water bath, condense and reflux for 6 hours, after the reaction is complete, a yellow substance precipitates out, filter, wash with absolute ethanol and water several times, and dry in vacuum . Finally, a yellow insoluble in ethanol, soluble in water complex, dihydromyricetin Zn 2+ Complex 80mg, Dihydromyricetin Cu 2+ Complex 78.6mg, Dihydromyricetin Ni 2+ Complex 75.6 mg.

[0032]The complexes of dihydromyricetin reacted with Zn2+, Cu2+, and Ni2+ are all amorphous yellow powders, which are 312°C, 308°C, and 3...

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Abstract

The invention discloses an application of dihydromyricetin and a complex thereof in preparation of anti-cancer drugs. Composition containing dihydromyricetin has the function of inhibiting growth andproliferation of tumor cells, particularly, kidney cancer cells and can be used for development and application of anti-tumor drugs and tumor chemoprevention drugs. The water solubility can be notablyimproved and the inhibition function on tumor cells can be enhanced through complexes of dihydromyricetin with Zn<2+>, Cu<2+>, Ni<2+> or some amino acids such as lysine and L-arginine.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of dihydromyricetin in the preparation of drugs for treating kidney cancer. Background technique [0002] Cancer is one of the major diseases that threaten human health and life. Malignant tumors grow fast and show invasive growth, prone to hemorrhage, necrosis, ulcers, and often accompanied by distant metastasis, resulting in body weight loss, weakness, anemia, loss of appetite, fever, and severe damage to organ functions, eventually causing The patient died. The incidence of cancer in my country is severe, and the morbidity and mortality are on the rise. According to reports, the number of cancer patients in China in 2012 was 3.065 million, accounting for about one-fifth of the global incidence and ranking first among the causes of death. Correspondingly, the incidence and mortality of cancer in the world are also on the rise. According to the latest statist...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00
CPCA61K31/352
Inventor 张奇胡海峰刘英吴春珍姜锦锦李彻
Owner 国药集团健康产业研究院有限公司
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