A kind of recombinant pectin methylesterase pmea and its coding gene and application

A pectin methylesterase and gene technology, applied in the field of genetic engineering, can solve the problems of slow reaction and destruction of pectin polymerization degree and the like
CN108130335BActive Publication Date: 2021-08-03GUANGDONG QIZHI BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGDONG QIZHI BIOTECHNOLOGY CO LTD
Publication Date
2021-08-03

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Abstract

The invention discloses a recombinant pectin methylesterase pmeA, its coding gene and application. The present invention is based on the amino acid sequence of Aspergillus niger pmeA published on GenBank, and then optimizes the codons according to the codon preference of the host bacteria, thereby obtaining a new nucleotide sequence of the pmeA gene such as SEQ ID NO.1 As shown, the amino acid sequence of the expressed recombinant pectin methylesterase is shown in SEQ ID NO.2. After the pmeA gene was connected with the pCuGAPGαAL expression vector, the recombinant plasmid pCuGAPGαAL‑pmeA was integrated into the genome of Candida utilis to realize the heterologous expression of the pmeA gene. The recombinant pectin methylesterase can efficiently catalyze the hydrolysis of methoxy group in pectin, reduce the degree of esterification of pectin, and can be used in the production of food, medicine and chemical industries.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and in particular relates to recombinant pectin methylesterase and its application. Background technique

[0002] Pectin is abundant in plants, mainly in the form of high methoxyl pectin. For low-methoxyl pectin, there are few natural plants available for extraction. So far, the most reported at home and abroad is the extraction of low-methoxyl pectin from sunflower. However, the small planting area of ​​sunflower and the influence of temperature and season are not conducive to the commercial development of low-ester pectin.

[0003] At present, the methods for producing low-ester pectin include: acid method, concentrated ammonia method, alkali method and pectin methylesterase enzymatic hydrolysis method. The disadvantage of the first two methods is that the reaction is slow; the alkali method has a fast rate of ester reduction, but it will destroy the degree of polymerization of pectin; pect...

Claims

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