Preparation method of seaweed alcohol-free beer based on marine microorganism enzyme and seaweed alcohol-free beer
A technology of non-alcoholic beer and marine microorganisms, which is applied in the brewing of beer, can solve the problem of seaweed smell and other problems, and achieve the effect of pure bitterness, obvious hop aroma and good drinkability.
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[0022] Embodiments of the present invention provide a method for preparing seaweed non-alcoholic beer based on marine microbial enzymes, comprising the following steps:
[0023] S1: Preparation of seaweed enzyme:
[0024] Select clean seaweed raw materials that meet food hygiene standards, rehydrate fresh seaweed raw materials or dry raw materials, cut them, add water at a ratio of 1:10 by weight and homogenize them, then transfer them to fermentation tanks, stir and sterilize, and cool down to 40± Store at 2°C for later use. Food grade Bacillus subtilis solid dry powder inoculum (10 10 CFU / g) is prepared into 10% bacterial suspension with sterile physiological saline before inoculation, Lactobacillus plantarum dry powder microbial agent (10 9 CFU / g) was prepared into 10% bacterial suspension with sterile physiological saline before inoculation. The sterilized fermentation substrate is kept at 40°C, and the prepared Bacillus subtilis suspension is aseptically inserted into ...
Embodiment 1
[0038] Step 1: Preparation of red algae enzyme: fresh red algae raw materials or dried red algae are rehydrated, cut, added water and homogenized, then transferred to a fermentation tank for sterilization, and the sterilized fermentation substrates are successively inserted into subtilis at different temperatures Bacillus suspension and Lactobacillus plantarum suspension are fermented, and the fermented seaweed enzyme is obtained by solid-liquid separation to obtain red algae enzyme;
[0039] Step 2: Preparation of non-alcoholic beer: Take a certain amount of malt and rice, of which malt accounts for 60%-70%, after crushing, saccharify according to the material-water ratio of 1: (3-4), and the saccharification temperature is 63-65 ℃, the saccharification time is 60-80min, after the saccharification is completed, filter the wort, and then boil for 45-75min, add hops during the boiling process, after the boiling, the desired wort is obtained, and its concentration is 10-13°P; tak...
Embodiment 2
[0043] The technical process is the same as in Example 1, except that the prepared seaweed enzyme is green algae enzyme, and the mass percentage of the added green algae enzyme is 1%.
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