The invention provides 0.0% vol alcohol-free Pilsner beer and a preparation method thereof, and belongs to the technical field of beer brewage. The problems that alcohol-free beer at present lacks thealcohol ester flavor of traditional beer, and has weak taste and outstanding acid sensation can be solved. According to the technical scheme, the 0.0% vol alcohol-free Pilsner beer is included, the alcoholic strength of the beer is smaller than 0.05% vol, amaroid is 20 EBC to 25 EBC, the original wort concentration is 4.5 plato degrees to 7.5 plato degrees, the carbon dioxide content is 0.50% mass to 0.60% mass, the total alcohol is larger than or equal to 15 mg/L, and the total ester is larger than or equal to 5 mg/L. the beer is prepared through the following steps that the Pilsner beer issequentially subjected to preheating, degassing, dealcoholization, cooling, carbon dioxide filling, post modification, filtering, stabilization and filling, and the 0.0% vol alcohol-free Pilsner beeris prepared.