Alcohol fermentation method

A technology of alcohol fermentation and saccharification, applied in fermentation, biofuels, etc., can solve problems such as insufficient starch conversion rate, achieve the effects of easy processing, convenient acquisition, and promotion of liquefaction

Inactive Publication Date: 2018-06-15
江苏金茂源生物化工有限责任公司
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the alcohol fermentation process, fermentable sugars are not only used by Saccharomyces cerevisiae to generate the main product alcohol, but also cell structure substances and other metabolic by-products will be synthesized. In addition, some sugars will remain in the fermentation broth, which cannot be fully utilized by Saccharomyces cerevisiae. This results in insufficient starch conversion during fermentation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of alcoholic fermentation method, comprises the following steps:

[0031] a, pulping, obtaining starchy raw materials, and mixing the starchy raw materials with mixing water to form a starch solution after pulverizing the starchy raw materials;

[0032] b, liquefaction, adding liquefaction enzyme to the starch solution to prepare a liquefaction solution;

[0033] c. Saccharification, adding glucoamylase to the liquefaction solution to prepare a saccharification solution;

[0034] d, fermentation, adding propionic acid to the saccharification solution, and then inoculating the seed liquid to carry out alcoholic fermentation.

[0035] Wherein: the starchy raw material in step a is at least one of cassava, corn, and wheat, and the starchy raw material after crushing is used for alcohol fermentation, which is convenient to obtain and easy to crush; the starchy raw material and the mixing water are mixed The ratio is 1:2~5, which can make the starch solution formed ...

Embodiment 2

[0051] A kind of alcoholic fermentation method, its concrete alcoholic fermentation process step is as follows:

[0052] a. Mix corn flour with mixing water, the ratio of material to water is 1:2~5, add α-amylase with high temperature resistance and liquefaction enzyme of 15~20u / g, stir well to form corn flour solution, and mix corn flour The solution is heated to 90-95°C and maintained for 90-140 minutes;

[0053] The ratio of material to water is set to 1:3, 20u / g of α-amylase is added, and the heating temperature is 90°C, and the maintenance time is 120 minutes.

[0054] b. Cool the corn flour solution in step a to 40-60°C, then add 120-160u / g glucoamylase, stir evenly, keep warm, and saccharify for 20-60 minutes to form a saccharified corn flour solution;

[0055] Set the temperature down to 60°C in step b, add 160u / g glucoamylase, and saccharify for 40 minutes to achieve full saccharification.

[0056]c. Lower the temperature of the saccharified corn flour solution in s...

Embodiment 3

[0060] A kind of alcoholic fermentation method, its concrete alcoholic fermentation process step is as follows:

[0061] a. Mix cassava flour, corn flour and wheat flour with mixing water, the ratio of material to water is 1:2~5, add high temperature resistant α-amylase with liquefaction enzyme of 15~20u / g, stir well and form mixed powder solution, heating the mixed powder solution to 90-95°C and maintaining it for 90-140 minutes;

[0062] In the above steps, set the ratio of material to water to 1:5, add 18u / g of α-amylase, and the heating temperature is 92°C, and the maintenance time is 140 minutes.

[0063] b. Lower the temperature of the mixed powder solution in step a to 40-60°C, then add 120-160 u / g of glucoamylase, stir evenly, keep warm, and carry out saccharification for 20-60 minutes to form a saccharified mixed powder solution;

[0064] Wherein, in step b, the temperature is lowered to 40° C., 140 u / g of glucoamylase is added, and the saccharification time is 20 mi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an alcohol fermentation method. The alcohol fermentation method comprises the following steps: a, slurrying: obtaining a starchy raw material, crushing the starchy raw material,and mixing with blending water to form a starch solution; b, liquefying: adding liquefying enzyme into the starch solution to obtain a liquefied solution, c, saccharifying: adding saccharifying enzyme into the liquefied solution to obtain a saccharified solution; d, fermenting: adding propanoic acid into the saccharified solution, and then inoculating with seed liquid for alcohol fermentation. Bythe alcohol fermentation method, through addition of the propanoic acid into the saccharified starch solution, the starch conversion rate during the alcohol fermentation can be increased to 90% or above; in addition, the added propanoic acid is low in cost, so that increase in the yield of alcohol and the profit thereof is facilitated.

Description

technical field [0001] The invention relates to the technical field of alcohol fermentation industry, in particular to an alcohol fermentation method. Background technique [0002] Due to the reduction of global oil reserves and the severe air pollution caused by the use of fossil energy, many countries around the world are actively developing the biomass fuel market. Compared with traditional fossil energy, fuel alcohol has the advantages of being clean and renewable. It can be directly used as vehicle fuel or added to gasoline (ethanol gasoline), and has been vigorously developed worldwide. [0003] Fuel alcohol is mainly produced by fermentation, and the general production process is: 1) Pretreatment of raw materials to obtain fermentable sugars; 2) Saccharomyces cerevisiae uses fermentable sugars to produce alcohol; 3) Fermentation liquid undergoes distillation-rectification-dehydration and other processes Obtain finished fuel alcohol. During the alcohol fermentation p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06
CPCC12P7/06C12P2201/00Y02E50/10
Inventor 薛宏光王柯瞿玮张建华陈浩
Owner 江苏金茂源生物化工有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap