Alcohol fermentation method
A technology of alcohol fermentation and saccharification, applied in fermentation, biofuels, etc., can solve problems such as insufficient starch conversion rate, achieve the effects of easy processing, convenient acquisition, and promotion of liquefaction
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Embodiment 1
[0030] A kind of alcoholic fermentation method, comprises the following steps:
[0031] a, pulping, obtaining starchy raw materials, and mixing the starchy raw materials with mixing water to form a starch solution after pulverizing the starchy raw materials;
[0032] b, liquefaction, adding liquefaction enzyme to the starch solution to prepare a liquefaction solution;
[0033] c. Saccharification, adding glucoamylase to the liquefaction solution to prepare a saccharification solution;
[0034] d, fermentation, adding propionic acid to the saccharification solution, and then inoculating the seed liquid to carry out alcoholic fermentation.
[0035] Wherein: the starchy raw material in step a is at least one of cassava, corn, and wheat, and the starchy raw material after crushing is used for alcohol fermentation, which is convenient to obtain and easy to crush; the starchy raw material and the mixing water are mixed The ratio is 1:2~5, which can make the starch solution formed ...
Embodiment 2
[0051] A kind of alcoholic fermentation method, its concrete alcoholic fermentation process step is as follows:
[0052] a. Mix corn flour with mixing water, the ratio of material to water is 1:2~5, add α-amylase with high temperature resistance and liquefaction enzyme of 15~20u / g, stir well to form corn flour solution, and mix corn flour The solution is heated to 90-95°C and maintained for 90-140 minutes;
[0053] The ratio of material to water is set to 1:3, 20u / g of α-amylase is added, and the heating temperature is 90°C, and the maintenance time is 120 minutes.
[0054] b. Cool the corn flour solution in step a to 40-60°C, then add 120-160u / g glucoamylase, stir evenly, keep warm, and saccharify for 20-60 minutes to form a saccharified corn flour solution;
[0055] Set the temperature down to 60°C in step b, add 160u / g glucoamylase, and saccharify for 40 minutes to achieve full saccharification.
[0056]c. Lower the temperature of the saccharified corn flour solution in s...
Embodiment 3
[0060] A kind of alcoholic fermentation method, its concrete alcoholic fermentation process step is as follows:
[0061] a. Mix cassava flour, corn flour and wheat flour with mixing water, the ratio of material to water is 1:2~5, add high temperature resistant α-amylase with liquefaction enzyme of 15~20u / g, stir well and form mixed powder solution, heating the mixed powder solution to 90-95°C and maintaining it for 90-140 minutes;
[0062] In the above steps, set the ratio of material to water to 1:5, add 18u / g of α-amylase, and the heating temperature is 92°C, and the maintenance time is 140 minutes.
[0063] b. Lower the temperature of the mixed powder solution in step a to 40-60°C, then add 120-160 u / g of glucoamylase, stir evenly, keep warm, and carry out saccharification for 20-60 minutes to form a saccharified mixed powder solution;
[0064] Wherein, in step b, the temperature is lowered to 40° C., 140 u / g of glucoamylase is added, and the saccharification time is 20 mi...
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