Method for determining titer of lumbrukinase in earthworm extraction intermediate by using near-infrared spectroscopy

Abstract

The invention discloses a method for determining the titer of lumbrukinase in an earthworm extraction intermediate by using near-infrared spectroscopy. A method for establishing a to-be-used model comprises the following steps: acquiring the near-infrared spectra of different earthworm extraction intermediate samples and detecting the titers of lumbrukinase in the different earthworm extraction intermediate samples by using a fibrin plate method; analyzing the near-infrared spectra of the earthworm extraction intermediate samples by using PCA, rejecting abnormal earthworm extraction intermediate samples, analyzing the near-infrared spectra of earthworm extraction intermediate samples without the abnormal samples by using a KS algorithm, and classifying a calibration set and a verificationset; carrying out pretreatment by using a combined first-order derivative SG15 point smoothing and standardization method; carrying out modeling variable selection on the pretreated spectra by using acombination of the five non-continuous variable selection algorithms consisting of SPA, UVE, CARS, GA and CC; and establishing a partial least square quantitative analysis model for the titer of lumbrukinase in the earthworm extraction intermediate.

Description

technical field [0001] The invention belongs to the technical field of quality control in the production process of traditional Chinese medicines, and relates to a method for rapidly determining the potency of lumbrokinase in earthworm extract intermediates based on near-infrared spectrum analysis technology. Background technique [0002] Near Infrared Spectroscopy (Near Infrared Spectroscopy, NIRS) is an electromagnetic radiation wave between visible light (Vis) and mid-infrared (MIR). The first non-visible region discovered in the absorption spectrum. The near-infrared spectrum region is consistent with the combined frequency of the vibration of hydrogen-containing groups (O-H, N-H, C-H) in organic molecules and the absorption region of all levels of frequency multiplication. By scanning the near-infrared spectrum of the sample, the hydrogen-containing groups of organic molecules in the sample can be obtained. The characteristic information of the cluster, and the use of ...

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Problems solved by technology

Due to the complex composition of traditional Chinese medicine products, when using near-infrared spectroscopy to establish a qualitative or quantitative analysis model of traditional Chinese medicine, it is necessary to go through a long period of exploration and exploration to extract the effective information established by the model. At present, near-infrared spectral analysis technology has been widely used in the quality monitoring of the production process of traditional Chinese medicine, but The existing literature reports lack the use of NIRS to study the potency of lumbrokinase, and the related enterprises of Dilong production cannot perform rapid quality control on the titer of lumbrokinase in the production process

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