Microfludic immunodetection chip and method of cardiac troponin protein I

A technique for cardiac troponin and immune detection, which is applied in chemical instruments and methods, biological testing, material inspection products, etc., can solve the problems of long time, high cost, complicated operation procedures, etc., and achieves shortened processing time and reduced reagent cost. , the effect of huge economic benefits

Active Publication Date: 2018-06-29
HUNAN YONGHE YANGGUANG SCI & TECH +1
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Problems solved by technology

The main disadvantages are: large sample consumption, complex operation procedures, high cost, long time-consuming, etc., and of...

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  • Microfludic immunodetection chip and method of cardiac troponin protein I
  • Microfludic immunodetection chip and method of cardiac troponin protein I
  • Microfludic immunodetection chip and method of cardiac troponin protein I

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Embodiment Construction

[0024] The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

[0025] refer to Figure 1-Figure 4 As shown, the present invention provides a microfluidic immunoassay chip 4 for cardiac troponin I, comprising a chip upper cover 21 and a chip 22 with a structure, the chip 22 with a structure is provided with a sample inlet 1, a sample Storage pool 2, microchannel 3, reagent B filling pool 6, high value calibration pool 7, low value calibration pool 8, reaction detection pool 9, waste liquid pool 10, storage pool 12, second pump 13 and first pump 17.

[0026] Described microchannel 3 comprises microchannel main road, microchannel branch road, and described sample reservoir 2, reaction detection pool 9 and waste...

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Abstract

The invention discloses a microfludic immunodetection chip and method of cardiac troponin protein I. In the microfludic immunodetection chip, a sample storage pool, a reaction detection pool and a waste-liquid pool are arranged on a microchannel main path in sequence; one end of the microchannel main path is provided with a sampling port, and the other end of the microchannel main path is connected with a first pump; one end of a microchannel branch path is communicated with a water storage pool by a second pump, and the other end of the microchannel branch path is connected with the microchannel main path between the reaction detection pool and the waste-liquid pool; the microchannel branch path is provided with a reagent B filling pool and a high-value calibration pool in sequence; a low-value calibration pool is connected with the high-value calibration pool in parallel; the microchannel branch path between the reagent B filling pool and the high-value calibration pool is also communicated with the microchannel main path between the sample storage pool and the reaction detection pool by a microchannel connecting branch path. The microfludic immunodetection chip and method disclosed by the invention have the advantages that the sample treating time is greatly shortened, the cost is reduced, the detection accuracy can also be improved and the functions of mobile detection andhousehold self-service type detection and the like are realized.

Description

technical field [0001] The invention relates to the technical field of biological detection, and more specifically relates to a microfluidic immune detection chip and method for cardiac troponin I. Background technique [0002] Cardiac troponin (cTn) is a regulatory protein of cardiomyocyte contraction, which is composed of subunits of three different genes: cardiac troponin T (cTnT), cardiac troponin I (cTnI) and cardiac troponin C ( cTnC). The detection of cTnI is the most sensitive among the three, so the detection accuracy is also the highest. It is difficult for conventional methods to achieve high accuracy and precision in the detection of this project. However, cTnI has very important clinical significance, which is mainly manifested in: (1) cTnI is not affected by skeletal muscle damage, and it appears earlier after the onset of the disease and lasts for a long time, with its unique myocardial specificity and diagnostic window period The advantage of being longer i...

Claims

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Application Information

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IPC IPC(8): G01N33/68B01L3/00
CPCB01L3/5027B01L2200/10B01L2300/0861B01L2300/0887G01N33/68
Inventor 沈林张华戈军肖长河余琼林陈远超
Owner HUNAN YONGHE YANGGUANG SCI & TECH
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