Microfludic immunodetection chip and method of cardiac troponin protein I

Abstract

The invention discloses a microfludic immunodetection chip and method of cardiac troponin protein I. In the microfludic immunodetection chip, a sample storage pool, a reaction detection pool and a waste-liquid pool are arranged on a microchannel main path in sequence; one end of the microchannel main path is provided with a sampling port, and the other end of the microchannel main path is connected with a first pump; one end of a microchannel branch path is communicated with a water storage pool by a second pump, and the other end of the microchannel branch path is connected with the microchannel main path between the reaction detection pool and the waste-liquid pool; the microchannel branch path is provided with a reagent B filling pool and a high-value calibration pool in sequence; a low-value calibration pool is connected with the high-value calibration pool in parallel; the microchannel branch path between the reagent B filling pool and the high-value calibration pool is also communicated with the microchannel main path between the sample storage pool and the reaction detection pool by a microchannel connecting branch path. The microfludic immunodetection chip and method disclosed by the invention have the advantages that the sample treating time is greatly shortened, the cost is reduced, the detection accuracy can also be improved and the functions of mobile detection andhousehold self-service type detection and the like are realized.

Description

technical field [0001] The invention relates to the technical field of biological detection, and more specifically relates to a microfluidic immune detection chip and method for cardiac troponin I. Background technique [0002] Cardiac troponin (cTn) is a regulatory protein of cardiomyocyte contraction, which is composed of subunits of three different genes: cardiac troponin T (cTnT), cardiac troponin I (cTnI) and cardiac troponin C ( cTnC). The detection of cTnI is the most sensitive among the three, so the detection accuracy is also the highest. It is difficult for conventional methods to achieve high accuracy and precision in the detection of this project. However, cTnI has very important clinical significance, which is mainly manifested in: (1) cTnI is not affected by skeletal muscle damage, and it appears earlier after the onset of the disease and lasts for a long time, with its unique myocardial specificity and diagnostic window period The advantage of being longer i...

AI Technical Summary

Problems solved by technology

The main disadvantages are: large sample consumption, complex operation procedures, high cost, long time-consuming, etc., and often require professional personnel to operate, which seriously hinders the application and promotion of ACS instant and rapid detection

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