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Trichothecium-roseum-resistant peptide as well as preparation method and application thereof

A preparative technology of trichothecene pink, applied in the field of peptides, can solve the problems of low stability of antimicrobial peptides, high production costs, and restrictions on the widespread use of antimicrobial peptides, and achieve the effect of good stability and high antibacterial activity

Inactive Publication Date: 2018-07-03
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low stability of antimicrobial peptides and high production costs, the widespread use of antimicrobial peptides is limited. Therefore, researchers are committed to developing new antimicrobial peptides with high stability and low toxicity.

Method used

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  • Trichothecium-roseum-resistant peptide as well as preparation method and application thereof
  • Trichothecium-roseum-resistant peptide as well as preparation method and application thereof
  • Trichothecium-roseum-resistant peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0047] An anti-pink trichothecene, the amino acid sequence of the peptide is Gly-Gly-Ser-Gly-Gly-Gly-Ser-Ser-Ser-Gly-Gly-Ser-Ile-Gly-Gly-Arg.

[0048] The preparation method of the anti-pink trichothecene comprises the following steps:

[0049] ⑴ Pick Bacillus pumilus ( B. pumilus ; The preservation number of Bacillus pumilus in the China General Microorganism Culture Collection Management Center is CGMCC NO.15031, the address of the preservation unit: China. Beijing. Institute of Microbiology, Chinese Academy of Sciences; preservation date: December 07, 2017) pure culture of the strain 1~2 loops were inoculated on LB liquid medium, and cultured with shaking at 37°C and 180 r / min for 2~3 days to obtain a fermentation broth, which was centrifuged at a rate of 10,000 r / min for 20 minutes to obtain a supernatant.

[0050] (2) The supernatant is sequentially subjected to macroporous adsorption resin chromatography, size exclusion chromatography and preparative reverse high perfo...

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Abstract

The present invention relates to a trichothecium-roseum-resistant peptide. The peptide has an amino acid sequence of Gly-Gly-Ser-Gly-Gly-Gly-Ser-Ser-Gly-Gly-Ser-Ile-Gly-Gly-Arg. Meanwhile, the presentinvention also discloses a preparation method and application of the trichothecium-roseum-resistant peptide. The trichothecium-roseum-resistant peptide provided by the present invention has the characteristics of good stability and high antibacterial activity through an antibacterial test and a stability test.

Description

technical field [0001] The present invention relates to a kind of polypeptide, relate in particular to a kind of resistance Trichothecene pink ( Trichothecium roseum ) peptide and its preparation method and application. Background technique [0002] Pink Trichothecenes ( T. roseum ) is a pathogenic fungus that cannot be ignored in postharvest diseases of fruits and vegetables. The pathogenic fungus can enter the product body through wounds during postharvest storage and transportation, and then cause rot. According to reports, in developed countries, 15% to 25% of fresh fruits and vegetables are lost to post-harvest rot, while in developing countries, due to the lack of storage and transportation refrigeration equipment, the loss rate of fruit and vegetable rot is as high as 20% to 50%. Chemical fungicides are usually used to effectively control diseases, but there are problems such as chemical fungicide residues, environmental pollution and pathogen resistance. Therefor...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K1/20C07K1/16C12P21/02A23L3/3526C12R1/07
CPCA23L3/3526C07K7/08
Inventor 贠建民严海娇
Owner GANSU AGRI UNIV
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