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Multigene prediction model of colorectal tumors based on ngs technology in stool DNA

A colorectal tumor and gene technology, which can be used in the determination/inspection of microorganisms, pretreatment of biological substances, bioreactors/fermenters for specific purposes, etc., can solve the problems of low patient compliance, poor specificity, and low sensitivity, etc. Accurate and specific diagnosis

Active Publication Date: 2021-12-28
SHANGHAI REALBIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, the methods used for colorectal cancer screening mainly include colonoscopy, fecal occult blood detection, etc., but there are deficiencies such as low sensitivity, poor specificity, or low patient compliance.

Method used

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  • Multigene prediction model of colorectal tumors based on ngs technology in stool DNA
  • Multigene prediction model of colorectal tumors based on ngs technology in stool DNA
  • Multigene prediction model of colorectal tumors based on ngs technology in stool DNA

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Embodiment 1

[0041] Select clinical samples with different course distributions of colonoscopy cases from Lanxi Red Cross Hospital, the Second Affiliated Hospital of Xi'an Jiaotong University, Xijing Hospital, and Tangdu Hospital from 2016 to 2017, including 77 cases of colorectal tumors and 204 cases of asymptomatic people stool samples. The above samples were subjected to gene capture and high-throughput sequencing, and their clinical and pathological information were collected at the same time. Finally, the genome sequencing results and clinical information were comprehensively analyzed to construct a colorectal tumor cell detection model.

[0042] (1) Gene capture and high-throughput sequencing

[0043] Fecal samples were subjected to gene capture and high-throughput sequencing in our laboratory (see figure 1 ). Extract the genomic DNA of the sample to be tested, and use the probe to capture the target genes KRAS and NDRG4; use the bisulfite conversion method to convert the NDRG4 gen...

Embodiment 2

[0065] Listed 6 cases of volunteers who were treated in Lanxi Red Cross Hospital, the Second Affiliated Hospital of Xi'an Jiaotong University, Xijing Hospital, and Tangdu Hospital, the process of applying the model to obtain the consistency judgment of the variable results measured by NGS technology.

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Abstract

The present invention presents methods and systems for identifying colorectal tumor cells. The method comprises: respectively obtaining the KRAS gene and the NDRG4 gene of the sample to be tested, and performing methylation treatment on the NDRG4 gene; respectively sequencing the KRAS gene and the methylated NDRG4 gene to obtain the KRAS gene Sequencing results and NDRG4 gene sequencing results; the gene sequence of the first target target position in the KRAS gene sequencing results is first compared with the first reference sequence, and the KRAS gene mutation rate is calculated; the NDRG4 gene sequencing results are The gene sequence of the second target target position in the second comparison is performed with the second reference sequence, and the NDRG4 gene methylation linkage ratio is calculated; based on the KRAS gene mutation rate and the NDRG4 gene methylation linkage ratio, the Whether there are colorectal tumor cells in the sample to be tested. The method and system of the invention can quickly, simply and specifically detect whether they are colorectal tumor cells, and have high sensitivity.

Description

technical field [0001] The invention relates to the field of molecular biology technology. Specifically, a multigene prediction model for colorectal tumors based on fecal DNA based on NGS technology. More specifically, the present invention relates to methods and systems for identifying colorectal tumor cells based on KRAS and NDRG4 genes. Background technique [0002] Colorectal cancer (CRC) is a common malignant tumor, including colon tumors and rectal tumors, also known as colorectal tumors, refers to malignant tumors that occur in the colon or rectum of the human lower gastrointestinal tract, and is one of the major cancers that threaten human health. First, its incidence rate ranks the third among male tumors and the second among female tumors in the world. In my country, with the development of society and economy, changes in residents' living habits and dietary structure, the incidence and mortality of colorectal tumors in my country continue to rise. According to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12M1/00C12M1/34
CPCC12Q1/6886C12Q2600/156C12Q2600/154
Inventor 毛瑞芳陈璟王云霞王君秦楠
Owner SHANGHAI REALBIO TECH CO LTD
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