Applications of anti-asparagine endopeptidase antibody in preparation of reagent used in diagnosis of pancreatic ductal adenocarcinoma
A technology of pancreatic ductal adenocarcinoma and asparagine, which is applied in the field of bioengineering, can solve the problems of lack of serum detection indicators and achieve obvious technical effects
Active Publication Date: 2018-07-06
HUZHOU CENT HOSPITAL
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Problems solved by technology
[0003] At present, there is a lack of effective serum detection indicators for the diagnosis and treatment of pancreatic ductal adenocarcinoma
Method used
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Experimental program
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Effect test
Embodiment 1
[0021] Embodiment 1 ELISA detection method:
[0022] Preparation before experiment:
[0023] Primary antibody preparation: 25 μl (goat anti-human AEP antibody) diluted in 5ml dilution buffer;
[0024] Secondary antibody preparation: 10 μl (horseradish catalase-labeled anti-goat secondary antibody) diluted in 4ml dilution buffer
[0025] experiment process:
[0026] 1. Take 30 μl of sample (here, serum exosome extract from patients with pancreatitis or pancreatic cancer) and coat it on a transparent 96-well plate.
[0027] 2. Seal the plate and incubate overnight at room temperature.
[0028] 3. The next day, the supernatant was discarded and washed 3 times with washing buffer. 5 minutes each time. After each wash, the liquid in the pores should be completely removed.
[0029] 4. Block with dilution buffer and incubate at room temperature for 2 hours.
[0030] 5. Discard the supernatant, and wash repeatedly 3 times with washing buffer. 5 minutes each time. After each w...
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The invention provides applications of anti-asparagine endopeptidase antibody in preparation of reagent used in diagnosis of pancreatic ductal adenocarcinoma. The invention also provides a kit containing the anti-asparagine endopeptidase antibody, and applications of the anti-asparagine endopeptidase antibody as a serum biological index in detection of pancreatic ductal adenocarcinoma. It is foundin research by the inventors of the invention that the content of asparagine endopeptidase in serum exosome of patients with pancreatic ductal adenocarcinoma is obvious higher than the content of asparagine endopeptidase in serum exosome of patients with pancreatitis, so that the content of asparagine endopeptidase in serum exosome can be taken as a biochemical index in diagnosis of pancreatic ductal adenocarcinoma. The kit can be used for accurate quantitative determination of the content of asparagine endopeptidase in samples to be measured. The invention possesses significant importance indetermination of curative effect of patients with pancreatic ductal adenocarcinoma and dynamic observation of disease conditions, and important effect is achieved in medical detection field.
Description
Technical field: [0001] The invention belongs to the field of bioengineering, and relates to a diagnostic reagent, in particular to the use of an anti-asparagine endopeptidase antibody in preparing reagents for diagnosing pancreatic ductal adenocarcinoma. Background technique: [0002] Pancreatic cancer is a highly malignant tumor of the digestive tract that is extremely difficult to diagnose and treat. About 90% of pancreatic cancers are ductal adenocarcinomas originating from the ductal epithelium. The incidence and mortality of pancreatic cancer have increased significantly in recent years. The 5-year survival rate is <1%, and it is one of the malignant tumors with the worst prognosis. The early symptoms of pancreatic cancer are not typical, including indigestion, abdominal pain, jaundice and other symptoms. It is more common in 40-70 years old, and more men than women. Therefore, the early diagnosis rate of pancreatic cancer is not high, and the surgical mortality ...
Claims
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Login to View More IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57438G01N33/57488G01N33/6854G01N33/6893
Inventor 严强
Owner HUZHOU CENT HOSPITAL