Solid substrate and medium to cultivate cordyceps militaris with effective diseases prevention function

A technology of solid substrate and Cordyceps militaris, applied in the biological field, can solve the problems of lax disinfection, pollution of bacteria, yeast and mold, production loss, etc., and achieve the effects of shortening the growth cycle, reducing diseases and increasing yield

Inactive Publication Date: 2018-07-13
辽宁省农业科学院大连生物技术研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Recently, Wang Chengshu from the Institute of Plant Ecology of the Chinese Academy of Sciences discovered for the first time that Cordyceps militaris can synthesize pentostatin, a drug with anti-cancer activity, and that cordycepin and pentostatin are jointly synthesized by the same gene cluster. Other types of Cordyceps cannot synthesize cordycepin and pentostatin. This achievement deeply promotes the research progress of Cordyceps militaris, and also makes Cordyceps militaris particularly attractive. It also ignores the control of technology and environment. If the sterilization is not good or the disinfection is not strict, it is easy to cause pollution such as bacteria, yeast and mold, which will cause serious losses to production. Looking for a medium with high efficiency and antibacterial function becomes a pupae New demand in the field of Cordyceps culture medium

Method used

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  • Solid substrate and medium to cultivate cordyceps militaris with effective diseases prevention function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) An effective disease-preventing Cordyceps militaris culture medium is prepared from the following parts by weight of raw materials: 85 parts of rice, 5 parts of silkworm pupa powder, and 10 parts of tussah leaf powder. The nutrient solution is prepared from the following raw materials by weight: 0.5 part of sucrose, 0.5 part of yeast extract, 0.3 part of silk peptide powder, 0.05 part of ferric ammonium citrate, 0.1 part of potassium dihydrogen phosphate, 0.05 part of magnesium sulfate, vitamin B 1 0.005 parts, 97 parts water. The mass ratio of solid substrate to nutrient solution is 1:1.1. Tussah leaf powder has a fineness of 40 mesh.

[0018] The preparation of a high-efficiency disease-preventing Cordyceps militaris culture medium is as follows: weigh 85 parts of rice, 5 parts of silkworm pupa powder, and 10 parts of tussah leaf powder according to the weight of the medium formula, mix well, and divide into canned bottles (1000ml Canned bottle, each bottle with a s...

Embodiment 2

[0025] (1) A highly effective disease-preventing Cordyceps militaris culture medium is prepared from the following parts by weight of raw materials: 92 parts of rice, 3 parts of silkworm pupa powder, and 5 parts of tussah leaf powder. The nutrient solution is prepared from the following raw materials by weight: 1.0 part of sucrose, 0.8 part of yeast extract, 0.4 part of silk peptide powder, 0.08 part of ferric ammonium citrate, 0.2 part of potassium dihydrogen phosphate, 0.1 part of magnesium sulfate, and vitamin B 1 0.008 parts, 98 parts water. The mass ratio of solid substrate to nutrient solution is 1:1.2. Tussah leaf powder has a fineness of 40 mesh.

[0026] The preparation of a high-efficiency disease-preventing Cordyceps militaris culture medium is as follows: weigh 92 parts of rice, 3 parts of silkworm pupa powder, and 5 parts of tussah leaf powder according to the weight of the medium formula, mix well, and divide into canned bottles (1000ml Canned bottle, each bottle w...

Embodiment 3

[0032] (1) An effective disease-preventing Cordyceps militaris culture medium is prepared from the following parts by weight of raw materials: 98 parts of rice, 1 part of silkworm pupa powder, and 1 part of tussah leaf powder. The nutrient solution is prepared from the following raw materials by weight: 1.5 parts of sucrose, 1.0 part of yeast extract, 0.5 part of silk peptide powder, 0.1 part of ferric ammonium citrate, 0.3 part of potassium dihydrogen phosphate, 0.15 part of magnesium sulfate, vitamin B 1 0.01 parts, 99 parts water. The mass ratio of solid substrate to nutrient solution is 1:1.3. Tussah leaf powder has a fineness of 40 mesh.

[0033] The preparation of an effective disease-preventing Cordyceps militaris culture medium is as follows: weigh 98 parts of rice, 1 part silkworm pupa powder, and 1 part tussah leaf powder according to the weight of the medium formula, mix well, and divide into canned bottles (1000ml Canned bottle, each bottle has a solid base mass of 4...

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Abstract

The invention discloses a solid substrate and a medium to cultivate cordyceps militaris with effective diseases prevention function, in particular relates to the technical field of cultivate cordycepsmilitaris. The adopted solid substrate is prepared by the materials with the following parts by weight: 85 to 98 parts of cereal, 1 to 5 parts of silkworm chrysalis powder and 1 to 10 parts of oak tree leaf powder. The medium consists of the solid substrate and nutrient solution. The mass ration of the solid substrate and the nutrient solution is 1:(1.1-1.6). The nutrient solution consists of sugar, yeast extract, silk peptide, ammonium ferric citrate, potassium dihydrogen, magnesium sulfate, thiamine and water. The medium added with oak tree leaf powder to cultivate cordyceps militaris can effectively inhibit the growth of contaminated bacteria in the growth of cordyceps militairis. As a result, the incidence of diseases is obviously reduced. The cultivated cordyceps militaris are neat and stout, of which the average yield is 21% higher than the cordyceps militaris cultivated in normal medium. In conclusion, the prospect of applying the solid substrate and the medium to cultivate cordyceps militaris is good.

Description

Technical field [0001] The invention belongs to the technical field of biotechnology, and specifically relates to a solid substrate and culture medium for cultivation of Cordyceps militaris with high efficiency and disease prevention. Background technique [0002] CordyCep Smilitaris (L.) Link, also known as Cordyceps militaris or Cordyceps militaris, is a rare edible and medicinal fungus with high nutritional value and medicinal effects. The Ministry of Health listed it as a new resource in 2009 Food, with the reduction of wild Cordyceps militaris resources, artificial cultivation of Cordyceps militaris has become a large-scale product, and its products have been accepted by more and more consumers. Recently, Wang Chengshu and others from the Institute of Plant Ecology of the Chinese Academy of Sciences discovered for the first time that Cordyceps militaris can synthesize pentostatin, a drug with anti-cancer activity, and synthesize cordycepin and pentostatin from the same gene ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20C05G3/00C05G1/00A01G18/00
CPCC05B7/00C05F1/00C05G3/00C05G5/20C05F11/00C05C3/00C05D5/00C05F11/08C05F11/10
Inventor 李亚洁温志新孟楠李学军马淑慧孙永欣米锐李树英都兴范
Owner 辽宁省农业科学院大连生物技术研究所
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