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Method for removing alloursodeoxycholic acid in duck bile

A technology of ursodeoxycholic acid and chenodeoxycholic acid, which is applied in the field of bioengineering, can solve the problems of affecting the conversion rate of chenodeoxycholic acid and ursodeoxycholic acid, and the effect is not significant, so as to broaden the sources of raw materials and sewage. The effect of controllable and improved utilization

Active Publication Date: 2018-07-20
CHANGDE YUNGANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the process of processing, in order to make the extraction of chenodeoxycholic acid more pure, people have adopted various methods to remove allocholic acid impurities in duck bile, but the effect is not significant
After years of research, it has been found that there is an impurity called isoursodeoxycholic acid in duck bile. The most difficult impurity to remove when purifying chenodeoxycholic acid will definitely affect the conversion rate when chenodeoxycholic acid is prepared from ursodeoxycholic acid

Method used

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  • Method for removing alloursodeoxycholic acid in duck bile
  • Method for removing alloursodeoxycholic acid in duck bile

Examples

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Embodiment 1

[0022] A method for removing isoursodeoxycholic acid in duck bile, the process steps are as follows:

[0023] (1) Preparation of crude chenodeoxycholic acid: take 500 kg of bile acid raw material of duck gall, and use butyl acetate magnesium salt method to obtain crude chenodeoxycholic acid. The wet weight of the crude product is 0.48%, and the crude product weighs 75.6 kg, the content of allocholic acid in the crude product is 0.8%, and the content of isoursodeoxycholic acid is 7.2%.

[0024] (2) Preparation of crystallization solution: Put 75.6 kg of crude chenodeoxycholic acid prepared in step (1) into a 2000L stainless steel kettle, add 453.6 kg of ethyl acetate with a water content of 0.2%, and then add 37.8 kg of ethyl acetate with a purity of 99.5%. Diol, stirred and heated to boiling, kept boiling and dissolved for 1 hour until the crude product was completely dissolved. Stand still for 1 hour to separate the lower water phase, and adjust the moisture of the upper org...

Embodiment 2

[0029] A method for removing isoursodeoxycholic acid in duck bile, the process steps are as follows:

[0030] (1) Preparation of crude chenodeoxycholic acid: take 550 kg of bile acid raw material of duck gall, and use butyl acetate magnesium salt method to obtain crude chenodeoxycholic acid. The wet weight of the crude product is 0.5%, and the crude product weighs 85.2 kg, the content of allocholic acid in the crude product is 0.83%, and the content of isoursodeoxycholic acid is 7.5%.

[0031] (2) Preparation of crystallization solution: Put 85.2 kg of crude chenodeoxycholic acid prepared in step (1) into a 2000L stainless steel kettle, add 511.2 kg of ethyl acetate with a water content of 0.15%, and then add 42.6 kg of ethyl acetate with a purity of 99.5%. Diol, stirred and heated to boiling, kept boiling and dissolved for 1 hour until the crude product was completely dissolved. Let stand for 1 hour to separate the lower aqueous phase, and adjust the moisture of the upper or...

Embodiment 3

[0036] A method for removing isoursodeoxycholic acid in duck bile, the process steps are as follows:

[0037] (1) Preparation of crude chenodeoxycholic acid: take 480 kg of bile acid raw material of duck gall, and obtain crude chenodeoxycholic acid by the butyl acetate magnesium salt method. The wet weight of the crude product is 0.58%, and the crude product weighs 73.2 kg, the content of allocholic acid in the crude product is 0.85%, and the content of isoursodeoxycholic acid is 7.3%.

[0038] (2) Preparation of crystallization solution: Put 73.2 kg of crude chenodeoxycholic acid prepared in step (1) into a 2000L stainless steel kettle, add 439.2 kg of ethyl acetate with a water content of 0.15%, and then add 36.6 kg of ethyl acetate with a purity of 99.5%. Diol, stirred and heated to boiling, kept boiling and dissolved for 1 hour until the crude product was completely dissolved. Let stand for 1 hour to separate the lower aqueous phase, and adjust the moisture of the upper o...

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Abstract

The invention discloses a method for removing alloursodeoxycholic acid in duck bile. The method comprises the processing steps of preparing a crude product of chenodeoxycholic acid, preparing crystalline liquid, carrying out crystallization to obtain chenodeoxycholic acid crystalline liquid, preparing a pure product of the chenodeoxycholic acid, and the like. After the method provided by the invention is adopted, not only can chenodeoxycholic acid in the duck bile be purified, but the alloursodeoxycholic acid with a pharmaceutical value also can be separated out, so that the utilization rate of bile acid resource is increased to the utmost extent. Furthermore, the method provided by the invention produces a small amount of residual wastes, and the residual wastes can be recycled by means of extraction, so that the loss of the bile acid resource is reduced to the utmost extent.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for removing isoursodeoxycholic acid in duck bile. Background technique [0002] People have a long history of research on cholic acid compounds. Driven by the rapid development of biochemistry, the research on cholic acid compounds is also deepening. Chenodeoxycholic acid is one of the most widely used drugs for treating gallstones in the world, and it is also the main raw material for the synthesis of ursodeoxycholic acid. The market demand for chenodeoxycholic acid and ursodeoxycholic acid continues to grow rapidly. In the case of increasing, in order to broaden the source of its raw materials, people began to extract chenodeoxycholic acid from duck bile with low cost and easy-to-obtain raw materials, so as to obtain more raw materials for preparing ursodeoxycholic acid. [0003] In the process of processing, in order to make the extraction of chenodeoxycholic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00
CPCC07J9/005
Inventor 邓家国雷平蔡宏军李国军
Owner CHANGDE YUNGANG BIOTECHNOLOGY CO LTD