A method for tissue culture and rooting of adventitious buds of saline-alkali-tolerant fast-growing Ulmus clonal
A saline-alkali-tolerant and clonal technology is applied in the field of tissue culture rooting of adventitious buds of saline-alkali-tolerant fast-growing white elm clones. , the effect of reducing the dosage
Active Publication Date: 2020-03-20
SHANDONG JIANZHU UNIV +1
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Problems solved by technology
But, because the adventitious buds of Ulmus clonale that are used for rooting in the bottle have strict requirements (the height of the seedling must be 2-3.5cm, robust, individuals with stretched leaves), it causes great waste in actual production
Activated carbon is very easy to precipitate when the medium solidifies, causing unnecessary troubles to the preparation of rooting medium
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[0032] (1) Selection of adventitious buds for rooting: select the adventitious buds of the saline-alkali-tolerant fast-growing Ulmus ultifolia clone (the growth period is less than one week) through normal growth, more than 1.5 cm in plant height, and through multiple successive cultures (or callus differentiation formation). except for tender tissues);
[0033] (2) Adventitious bud inoculation: Inoculate the adventitious buds of the saline-alkali-tolerant Ulmus clonal clone in EM rooting medium for rooting culture.
[0034] The growth environment is: 26°C (±2°C) during the day / 18°C (±2°C) at night, 12 hours of light per day, light intensity of 2000-3500 lux, and relative air humidity of 90%.
[0035] EM rooting medium includes the following components and their contents: CaCl 2 2H 2 O 220.5mg / L, NH 4 NO 3 800mg / L, KNO 3 1011mg / L, MgSO 4 1680.5mg / L, NaH 2 PO 4 2H 2 O 156mg / L, K 2 SO 4 175mg / L, Na 2 SO 4 150mg / L, CaCl 2 115mg / L, CoCl 2 ·6H 2 O 0.24mg / L, Cu...
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The invention belongs to the technical field of plant culture and particularly relates to a tissue culture and rooting method of saline-alkaline tolerant fast-growing ulmus pumila clonal line adventitious buds. The saline-alkaline tolerant fast-growing ulmus pumila clonal line adventitious bud tissue culture and rooting method comprises selection and inoculation of saline-alkaline tolerant fast-growing ulmus pumila clonal line adventitious buds, wherein the rooting conditions of the adventitious buds are as follows: rooting is conducted in a formula (EM rooting+IBA1mg / L+2 percent S) researchedand developed by the invention, the culture temperature is 26 DEG C (+ / -2) DEG C in daytime and 18 DEG C (+ / -2) DEG C at night, cultivation is conducted in the environment with the relative air humidity of 90 percent, illumination is conducted for 12 hours per day, and the illumination intensity is 2000 to 3500 lux. By adoption of the formula and the method, the rooting rate of the ulmus pumila adventitious buds reaches to 96 percent; furthermore, the utilization rate of the adventitious bud materials for rooting is increased by more than 20 to 35 percent compared with that in earlier stage.
Description
technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for tissue culture and rooting of adventitious buds of saline-alkali-tolerant fast-growing Ulmus ulmus clones. [0002] technical background [0003] White elm (Ulmus pumila L.), a plant of the genus Ulmus in the family Ulmaceae, has many varieties. [0004] Saline-alkali land is a type of land where salt accumulates in the soil and the salt contained in the soil has affected the normal growth of plants. The area of saline-alkali land in my country is 3.5×10 7 hm 2 , equivalent to 33.3% of the arable land area. How to effectively use the ecological environment in saline-alkali areas and increase the coverage of woody plants in saline-alkali areas is one of the important problems faced by forestry workers. As one of the main ornamental tree species in gardens in my country, white elm is highly resistant to toxic gases such as smoke, sulfur dioxi...
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 慕德宇梁玉王强范小莉房用慕宗昭林大为冒文娟
Owner SHANDONG JIANZHU UNIV