A new method for simple and convenient detection of SNPs

Abstract

The invention discloses a new method for simple and convenient detection of SNP. The primer set provided by the present invention; primer set I includes two upstream primers and one downstream primer; the difference between the FX1 primer and the FY1 primer: the first nucleotide at the 3' end, the FX1 primer corresponds to the X polymorphism of the specific SNP, FY1 The primers correspond to the Y polymorphism; FX1 primers and FY1 primers are both base mismatch primers; primer set II includes two upstream primers and one downstream primer; the difference between FX2 primers and FY2 primers: the first nucleotide at the 3' end is different, The FX2 primer corresponds to the X polymorphism of the specific SNP, and the FY2 primer corresponds to the Y polymorphism; 10-20 random nucleotides are added to the 5' end of the FY2 primer; both the FX2 primer and the FY2 primer are base mismatch primers. The primer set provided by the invention can be widely used in the detection of various target SNP sites, and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a new method for simple and convenient detection of SNP. Background technique [0002] The genetic information of life is stored in the DNA sequence, and the entire DNA of each cell constitutes the genome of the organism. Genomic DNA sequence variation is the basis of species genetic diversity, and single nucleotide variation is the most basic variation form of DNA sequence. With the rapid development of whole genome sequencing technology, a large number of single nucleotide polymorphisms (singlenucleotide polymorphisms, SNP) data have been mined, widely used as genome characteristic markers, and become the third generation of molecular breeding mark. [0003] SNP markers are developed based on single nucleotide polymorphisms, which mainly refer to DNA sequence polymorphisms caused by single nucleotide variations at the genomic level. The number of SNP markers is large...

AI Technical Summary

Problems solved by technology

Whole-genome sequencing and SNP chip hybridization are the most efficient and direct methods for discovering SNPs, but these two methods require large instruments and highly skilled technicians, and are suitable for high-throughput detection of multiple SNP sites

Taqman probe method, competitive allele-specific (KASP) detection and high-resolution melting curve (HRM) can realize the detection of the determined target SNP site through the Real-Time PCR instrument, but these three methods require The use of fluorescent dyes to achieve SNP typing, compared with ordinary PCR, is still more expensive, and these three detection methods have higher requirements for DNA quality, DNA concentration, and the technical level of instrument operators, and slight sample contamination or concentration fluctuations Inhomogeneity can lead to false positives, leading to poor repeatability of some results and affecting the detection conclusion

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