Xinjiang artemisia plant metabonomics analysis method

A metabolomics and analysis method technology, which is applied in the field of metabolomics analysis of Artemisia japonica plants, can solve the problems of comprehensively characterizing the pharmacodynamic material basis of Artemisia japonicus, time-consuming separation and extraction process, and limited analytical throughput. Achieve high sensitivity, easy operation and good stability

Active Publication Date: 2018-07-27
MINZU UNIVERSITY OF CHINA
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Problems solved by technology

However, these studies mostly use traditional analysis methods, the separation and extraction process is time-consuming, the analysis throughput is limited, and only for a few compounds, it is impossible to efficiently, systematically and comprehensively characterize the pharmacodynamic substance basis of Artemisia argyi

Method used

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  • Xinjiang artemisia plant metabonomics analysis method
  • Xinjiang artemisia plant metabonomics analysis method
  • Xinjiang artemisia plant metabonomics analysis method

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Experimental program
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Embodiment 1

[0043] 1. Experimental part

[0044] Sample collection and preparation:

[0045] Artemisia sativa in full flowering period was collected from Fuyun County, Xinjiang Uygur Autonomous Region (artificially cultivated species, seedlings were raised in August 2014, and transplanted to the field from the end of June to the beginning of July 2015). Immediately after collection, it was placed in an insulated box with dry ice Temporarily store and transfer to -80°C freezer as soon as possible. The roots, stems, branches, leaves, and flowers of Artemisia argyi in Xinjiang were taken, ground into powder in liquid nitrogen with a mortar, and the powder was placed in a freeze dryer for 48 hours. Weigh 50 ± 1 mg of freeze-dried powder in a homogenization tube, use 1 mL of methanol-water (80:20, V / V) to extract in a high-speed disperser for 3 min under ice bath conditions (25000r / min, 1min; 3000r / min, 1min; 25000r / min, 1min), ultrasonicated for 10min, centrifuged for 10min (13500r / min), pl...

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Abstract

The invention discloses a Xinjiang artemisia plant metabonomics analysis method. The method comprises the following steps: 1) taking dry powder of different tissue organs of Xinjiang artemisia, respectively performing steps of extraction with an organic solvent, ultrasonic wave, centrifugation, and condensation to obtain a concentrate, then performing redissolving, ultrasonic wave, centrifugationand filtering on the concentrate, and taking supernatants of metabolome of the different tissue organs; 2) employing a liquid chromatogram-tandem mass spectrometry on the supernatants of metabolome ofthe different tissue organs, and processing the obtained data; employing multivariable statistics analysis to obtain differential variables, according to a high-resolution mass-to-charge ratio and tandem mass spectrum data of the differential metabolite, and combining a metabolite database Metlin retrieval contrast analysis, obtaining the difference of the plant metabolome of the different tissueorgans of the Xinjiang artemisia. The method has the advantages that pre-treatment operation on a sample is simple, the stability of an analysis method is good, sensitivity is high, a metabolite covering range is wide, and the metabolome information of the plant can be efficiently and comprehensively obtained.

Description

technical field [0001] The invention relates to an analysis method of plant metabolomics of Artemisia sativa plant, in particular to an analysis method of plant metabolomics of Artemisia spp. Xinjiang based on liquid chromatography-tandem mass spectrometry technology, and belongs to the field of plant metabolomics analysis. Background technique [0002] Searching for active substances and discovering new drug source molecules from medicinal plants has always been an important part of new drug research. Medicinal plants are diverse in species and contain a wide variety of chemical components, which provide abundant resources for the development of new drugs. However, the traditional research methods for the active components of medicinal plants are difficult to fully reflect the chemical components and their distribution in the body, and the influence of the ecological environment on their substances in the body. Plant metabolomics research starts from the whole and systemat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 王中华陈路路周帜何秉淑再帕尔·阿不力孜
Owner MINZU UNIVERSITY OF CHINA
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