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Anthraquinone compound, preparation method thereof, and application to preparation of drug for treating cancer

A technology of anthraquinones and compounds, which is applied in the field of preparation of active compounds from secondary metabolites of actinomycetes, can solve the problems of decreased rate of discovery and waste of manpower, material and financial resources, etc., and achieve the effect of easy cultivation

Inactive Publication Date: 2018-08-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the 1990s, the new rate of natural products from land-based sources has declined year by year, and the compounds that have been repeatedly isolated are a serious waste of manpower, material and financial resources. People have gradually shifted their attention to natural products from marine sources.

Method used

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  • Anthraquinone compound, preparation method thereof, and application to preparation of drug for treating cancer
  • Anthraquinone compound, preparation method thereof, and application to preparation of drug for treating cancer
  • Anthraquinone compound, preparation method thereof, and application to preparation of drug for treating cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Seed liquid

[0041] Streptomyces rimosus subsp. Paromomycinus NRRL 2455 was inoculated into a 500 mL Erlenmeyer flask containing 250 mL of Gao's No. 1 liquid medium, and cultured with shaking in a shaker (28° C., 180 rpm) for 3-5 days to obtain a seed solution.

[0042] 2. Fermentation

[0043] The above-mentioned seed liquid was inoculated into a rice solid medium in an amount of 10 mL inoculated per bottle, and the fermentation was terminated after standing culture at 28°C for 30 days.

[0044] 3. Rough mention

[0045] Each bottle of solid fermented product was soaked in about 300 mL of EA (ethyl acetate) overnight, the filtrate was collected, and concentrated under reduced pressure to obtain a crude extract (oily extract).

[0046] 4. Compound separation

[0047] (a) The crude extract is purified by silica gel column chromatography, and then is eluted with a gradient of dichloromethane-methanol system with a methanol volume percentage of 0% to 100%; after the components con...

Embodiment 2

[0058] 1. Seed liquid

[0059] Streptomyces rimosus subsp. Paromomycinus NRRL 2455 was inoculated into a 500 mL Erlenmeyer flask containing 250 mL of Gao's No. 1 liquid medium, and cultured with shaking in a shaker (28° C., 180 rpm) for 3-5 days to obtain a seed solution.

[0060] 2. Fermentation

[0061] The above-mentioned seed liquid was inoculated into a rice solid medium in an amount of 10 mL inoculated per bottle, and the fermentation was terminated after standing culture at 28°C for 30 days.

[0062] 3. Rough mention

[0063] Each bottle of solid fermented product was soaked in about 300 mL of EA (ethyl acetate) overnight, the filtrate was collected, and concentrated under reduced pressure to obtain a crude extract (oily extract).

[0064] 4. Compound separation

[0065] (a) The crude extract is purified by silica gel column chromatography, and then is eluted with a gradient of dichloromethane-methanol system with a methanol volume percentage of 0% to 100%; after the components con...

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Abstract

The invention discloses an anthraquinone compound having the formula shown in the following formula (I) or formula (II). The anthraquinone compound with a novel structure is a natural product obtainedby fermenting and purifying rice by actinomycetes, has obvious anti-tumor activity, especially has significant proliferation inhibition for human prostate cancer cell line PC3 cells and human colon cancer cell line SW620 cells, and can be used for developing anti-tumor drugs. (The formulas are shown in the description.).

Description

Technical field [0001] The invention relates to the field of preparation of active compounds from secondary metabolites of actinomycetes, in particular to a class of anthraquinone compounds, a preparation method thereof, and application in the preparation of cancer drugs. Background technique [0002] Due to its unique natural environment and unique secondary metabolites of marine organisms, marine natural products have become a hot topic in recent years. The demand for clinical drugs is shifting from terrestrial natural products to marine natural products. It can be seen that marine natural products are facing huge challenges and pressures with the mission of new drug development. [0003] For a long time, natural products extracted from terrestrial animal and plant microorganisms are important sources of clinical drugs. For example, the analgesic morphine is extracted from poppies, the cardiotonic digitoxin is derived from digitalis, and the anti-cancer drug paclitaxel It is obt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/08C07C50/34A61P35/00A61P35/02C12P7/66C12R1/59
CPCA61P35/00A61P35/02C07C50/34C07D493/08C12P7/66
Inventor 马忠俊丁婉婧王倩倩嵇媛媛
Owner ZHEJIANG UNIV
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