Calibration method of biological particle counter and calibration device of biological particle counter
A technology of biological particles and calibration methods, which is applied in the fields of biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., and can solve problems such as difficulty and easy change of fluorescence intensity
Active Publication Date: 2022-03-01
RION COMPANY
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Problems solved by technology
[0010] In addition, it is also conceivable to form calibration particles by mixing a fluorescent agent in a base material so that the fluorescence intensity increases correspondingly to the biological particles to be counted. The intensity is easy to change, so it is difficult to control the amount of fluorescent agent so that the fluorescence intensity can be kept constant at the desired value, and it is difficult to manufacture particles for calibration
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Embodiment Construction
[0018] Embodiments of the present invention will be described below with reference to the drawings.
[0019] figure 1 It is a block diagram showing the configuration of the calibration device of the bioparticle counter according to the embodiment of the present invention. figure 1 The calibration of the biological particle counter 1 shown is the calibration method of the biological particle counter according to the embodiment of the present invention, which is automatically executed by the calibration device 2 .
[0020] The biological particle counter 1 includes, for example, the device described in the above-mentioned Japanese Patent Laid-Open No. 2013-117466: a flow cell (not shown) in which a sample liquid containing biological particles or calibration particles flows; A light source and an optical system (not shown) that irradiate laser light in the measurement area; an optical system (not shown) that separates the fluorescence emitted by the particles (biological partic...
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Abstract
The present invention can accurately correct the bioparticle counter according to the bioparticles to be counted. A bioparticle counter (1) has a first circuit (11) that generates a first signal corresponding to fluorescence and scattered light emitted by bioparticles in response to irradiation light. A measured value acquiring unit (22) acquires a voltage value of a first signal of a calibration particle with a fluorescent agent infiltrated inside a surface of a polystyrene particle from a bioparticle counter (1). The circuit adjustment part (23) uses the reference data (31 ), determine the reference voltage value (V1r) of the first signal corresponding to the fluorescent agent parameter value of the calibration particles used for calibration, and make the voltage value (V1) of the first signal of the calibration particles as the reference The first circuit (11) is adjusted by means of a voltage value (V1r).
Description
technical field [0001] The invention relates to a calibration method for a biological particle counter and a calibration device for a biological particle counter. Background technique [0002] In a certain bioparticle counter, a liquid containing bioparticles is made to flow into a flow cell, a predetermined measurement area in the flow cell is irradiated with laser light, and scattered light and autofluorescence from bioparticles passing through the measurement area are received. Furthermore, based on the detection signal of scattered light and the detection signal of fluorescence, the biological particles are counted by distinguishing them from non-biological particles, and the particle diameter of the biological particles passing through the measurement area is measured based on the detection signal of scattered light, and the number of biological particles passing through the measurement area is measured based on the detection signal of fluorescence. , to measure the flu...
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IPC IPC(8): C12M1/34G01N15/06G01N21/64
CPCG01N21/278G01N15/1012G01N21/64
Inventor 关本一真大桥勇贵三宫尚志中岛勉福田美幸上野胜植屋佑一
Owner RION COMPANY