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Method of using composite flocculant to flocculate and collect Chlorella pyrenoidosa

A technology of Chlorella pyrenoidosa and a flocculant is applied in the field of flocculation and harvesting of Chlorella pyrenoidosa using a combined flocculant, which can solve the problems of high cost and difficulty in harvesting of Chlorella pyrenoidosa, and achieve the flocculation effect. Reliable, improve flocculation speed and reduce dosage

Inactive Publication Date: 2018-08-10
JIANGXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of high cost and difficult harvesting of chlorella pyrenoidosa at present, the present invention provides a method for flocculating and harvesting chlorella pyrenoidosa by using a combined flocculant

Method used

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  • Method of using composite flocculant to flocculate and collect Chlorella pyrenoidosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) The Chlorella pyrenoidosa used in this example is an alga species preserved by the Microalgae Breeding and Deep Processing Engineering Technology Center of Jiangxi Province. The medium is prepared with tap water, and the Chlorella pyrenoidosa medium is the medium optimized by the Engineering Center.

[0032] (2) Add the chlorella species to the chlorella culture medium with a pH of 6, the initial OD 680 The temperature was adjusted to 0.3, and the temperature was 25° C., the light system was light:dark=12h:12h, and the cultured in a light incubator with a light intensity of 3 000 lx for 4 days.

[0033] (3) will OD 680 300 milliliters of the chlorella pyrenoidosa liquid that is in the growth logarithmic phase of 1.0 is placed in a 500 milliliter triangular flask, and 50 milligrams of combined flocculants (carboxymethyl chitosan: inulin: food-grade polyaluminium=950 :10:1).

[0034] (4) Stir for 5 minutes with a mechanical stirrer at a rotating speed of 50rpm, then...

Embodiment 2

[0036] (1) The Chlorella pyrenoidosa used in this example is an alga species preserved by the Microalgae Breeding and Deep Processing Engineering Technology Center of Jiangxi Province. The medium is prepared with tap water, and the Chlorella pyrenoidosa medium is the medium optimized by the Engineering Center.

[0037] (2) Add the Chlorella species to the Chlorella pyrenoidosa medium with a pH of 7, the initial OD 680 The temperature was adjusted to 0.4, and the temperature was 30° C., the light system was light:dark=16h:8h, and the cultured in a light incubator with a light intensity of 4000 lx for 4 days.

[0038] (3) will OD 680 300 milliliters of the chlorella pyrenoidosa liquid in the growth logarithmic phase of 1.1 is placed in a 500 milliliter triangular flask, and 50 milligrams of combined flocculants are added (carboxymethyl chitosan: inulin: chitosan: food grade Polyaluminum = 950:20:50:5).

[0039] (4) Use a mechanical stirrer at a rotating speed of 20rpm to stir ...

Embodiment 3

[0041] (1) The Chlorella pyrenoidosa used in this example is an alga species preserved by the Microalgae Breeding and Deep Processing Engineering Technology Center of Jiangxi Province. The medium is prepared with tap water, and the Chlorella pyrenoidosa medium is the medium optimized by the Engineering Center.

[0042] (2) Add the Chlorella species to the Chlorella pyrenoidosa medium with a pH of 8, the initial OD 680 The temperature was adjusted to 0.35, and the temperature was 30° C., the light system was light:dark=12h:12h, and the cultured in a light incubator with a light intensity of 5 000 lx for 3 days.

[0043] (3) will OD 680 300 milliliters of the chlorella pyrenoidosa liquid in the growth logarithmic phase of 0.9 is placed in a 500 milliliter conical flask, and 50 milligrams of combined flocculants (carboxymethyl chitosan: chitosan: food-grade polyaluminum = 900:100:0.1).

[0044](4) Use a mechanical stirrer at a rotating speed of 40rpm to stir for 5 minutes, then...

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Abstract

The invention provides a method of using a composite flocculant to flocculate and collect Chlorella pyrenoidosa and belongs to the technical field of aquaculture. The method is characterized in that the composite flocculant that is millionth to ten thousandth of chlorella by volume is added into chlorella liquid that grows well and that has OD680 light absorption value of 0.9-1.2; pH is adjusted to 6-10; stirring is performed at 20-100 rpm for 5-20 min; standing is allowed for 0.5-8 h so that chlorella settles in floc form; supernate is discharged for chlorella culture; chlorella concentrate is collected; centrifuging is performed to collect chlorella; lyophilizing is carried out to obtain chlorella capable of serving as food or feed. The method has the advantages that the usage of the flocculant is decreased by adjusting pH of a flocculating system, the flocculating time is shortened by adding little polymerized aluminum into the biological flocculant, flocculating rate is higher than90%, the collected chlorella has good safety and reliability and is usable as food and animal feed, and the method is applicable to the industrial harvesting of chlorella.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for flocculating and harvesting chlorella pyrenoidosa by using a combined flocculant. Background technique [0002] Chlorella is the fastest growing animal and plant in the world, it can grow 4 times in 20 hours, mainly because it contains unique growth factors. It is rich in protein, various pigments, B vitamins, essential amino acids, trace elements and some biologically active metabolites. It has a series of physiological health functions such as anti-cancer, anti-radiation, anti-infection, anti-oxidation, prevention and treatment of hyperlipidemia, prevention and treatment of constipation and bone marrow suppression. Chlorella is a high-quality green nutrient source food, an important health product, and contains a unique CGF factor. It is known as the most ideal food for human beings in the 21st century and special food for astronauts. It is mainly used in food...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/02C12R1/89
CPCC12N1/02C12N1/12
Inventor 段敏静梁长利梁群张卿洋任嗣利
Owner JIANGXI UNIV OF SCI & TECH