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Pig mycoplasma pneumonia inactivated vaccine and preparation method thereof

A technology of inactivated vaccine, Mycoplasma hyopneumoniae, which is applied in the field of inactivated vaccine against Mycoplasma hyopneumoniae and its preparation, can solve the problems of difficult vaccine development and difficult estimation of vaccine protection rate

Inactive Publication Date: 2018-08-14
北京万牧源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This has brought great difficulties to the development of vaccines.
[0007] However, from clinical trials and the application of vaccines, it has been found that there is still no vaccine that can achieve a very satisfactory preventive effect. Pigs and breeding boars, intramuscular inoculation, immunization twice, safe for pigs, no side effects, compared with non-immune control pigs, the incidence of pneumonia in immunized pigs can be reduced by 50% to 60%, and the duration of immunity is 6 months

Method used

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  • Pig mycoplasma pneumonia inactivated vaccine and preparation method thereof
  • Pig mycoplasma pneumonia inactivated vaccine and preparation method thereof
  • Pig mycoplasma pneumonia inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment 1——vaccine preparation

[0074] 1. Seed Preparation for Production

[0075] After unsealing the freeze-dried strains, dissolve them in the culture medium, inoculate the modified Goodwin’s A26 liquid medium, and culture at 37°C for 3 to 7 days, until the color of the culture medium turns yellow, becomes slightly turbid, and the pH of the bacterial solution drops to 6.8 At about 10% of the inoculum size, continue for 1 generation, culture at 37°C for 3 to 7 days, and harvest when the color of the medium turns yellow, becomes slightly turbid, and the pH value of the bacterial solution drops to about 6.8. After passing the pure inspection, as a primary seed. Take the first-grade seed bacteria solution and inoculate it in the improved Goodwin's A26 liquid medium according to the inoculum amount of 10%. Shake culture at 37°C for 3 to 7 days, and harvest when the color of the medium turns yellow, slightly turbid, and the pH value of the bacterial solution drops to...

Embodiment 2

[0094] Embodiment 2 - the safety test of vaccine

[0095] Three batches of Mycoplasma hyopneumoniae inactivated vaccines were prepared, which were inactivated by BEI and then concentrated. Montanide GEL01RP water-soluble adjuvant was added to make three batches of inactivated vaccines against Mycoplasma hyopneumoniae. The batch numbers were 001, 002 and 003, respectively. The product has been tested for safety. Prepare 3 batches of vaccines, single-dose inoculation to the minimum use age piglets, single-dose re-inoculation to piglets, single-dose over-dose inoculation to piglets, single-dose re-inoculation to pregnant animals, over-dose re-inoculation to pregnant animals, The safety of single-dose inoculation of animals and over-dose inoculation of breeding boars was tested. The results showed that: during the observation period, the spirit and food intake of the experimental animals were normal, and the body temperature after inoculation was normal without significant changes...

Embodiment 3

[0096] Embodiment 3——The immunity test of vaccine

[0097] The following immune efficacy tests were carried out with the prepared 3 batches of vaccines: the immune efficacy test of the inactivated mycoplasma pneumoniae vaccine on the target animal piglets; the immune efficacy test of the inactivated mycoplasma pneumoniae vaccine on the target animal pregnant sows. The test results are as follows:

[0098] (1) The immune efficacy test of the inactivated Mycoplasma pneumoniae vaccine on piglets aged 2 to 3 weeks, the piglets aged 2 to 3 weeks were inoculated intramuscularly with the inactivated Mycoplasma pneumoniae vaccine, 2.0ml / head, followed by the same dosage and route 21 days later. Strengthen the immunization, 35 days after the second immunization, the immunization group and the control group were intratracheally injected with 5.0ml of virulent Mycoplasma hyopneumoniae virulent ZY strain-containing diseased lung tissue suspension to challenge the virus, and observed for 2...

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Abstract

The invention relates to a production method for pig mycoplasma pneumonia inactivated vaccine. The method takes an isolated mycoplasma hyopneumoniae (Mycoplasma hyopneumoniae Mhp) ZY strain with strong poison immunity and good epidemic focus as a production bacterial strain, and efficient culture medium, appropriate cultural method, inactivation technology and appropriate immunologic adjuvant areused to prepare the mycoplasma hyopneumoniae inactivated vaccine. The production method for the pig mycoplasma pneumonia inactivated vaccine has the advantages that the content CUU of inactivated antigens is no less than 1.5*108 per dose in the prepared vaccine, the use of the vaccine is safe, healthful 2-3-week-old piglets easy to be infected do not have local or whole-length untoward effect after inoculation and all live healthily; the vaccine comprises an effectiveness testing result with a matched group by using an assay of vaccination and challenge method, and the decrement rate of pneumonia of tested pigs in the matched group is no less than 75%; the vaccine is still safe and effective after being stored at 2-8 DEG C for 27 months; and the effectiveness testing of the vaccine also can be performed by using an IHA method to detect the titer of a rabbit serum mycoplasma hyopneumoniae antibody, which has great benefit for the effectiveness testing of the vaccine.

Description

technical field [0001] The invention relates to an inactivated vaccine of swine mycoplasma pneumoniae and a preparation method thereof, belonging to the field of veterinary products. Background technique [0002] Mycoplasma Pneumonia of Swine (MPS), also known as Enzootic Pneumonia of Pig, is a contact and chronic respiratory infectious disease of pigs caused by Mycoplasma hyopneumoniae (Mhp). The main manifestations are cough and wheezing, shortness of breath and mouth breathing when the condition is exacerbated. The lesion is characterized by confluent bronchopneumonia with "meaty" or "pancreatic" parenchyma in the apical, cardiac, intermediate, and anterior thirds of the diaphragm. The disease has a worldwide distribution and is present in almost all countries. With the occurrence of swine panting disease, the growth rate of sick pigs will be reduced by 12-15%, the feed conversion rate will be reduced by 10%, and the fattening period will be extended by 1 month. Syndrom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02A61P11/00A61P31/04C12N1/20C12R1/35
CPCA61K39/0241A61K2039/521A61K2039/552A61P11/00A61P31/04C12N1/20
Inventor 宋艳丽朱萍王宝辉徐桓鹿钟文韩娥郭利敏司香玲
Owner 北京万牧源生物科技有限公司
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