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Totally enclosed type magnetic separation system

A magnetic bead and magnetic field technology, applied in the field of magnetic separation, can solve the problems of poor applicability, pollution, false positives, etc., and achieve the effect of wide application range and avoiding cross-contamination

Inactive Publication Date: 2018-08-14
冷彦宁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to separation, there are multiple washing processes in practical applications, so magnets or pipettes are required to enter and exit the reaction vessel multiple times, so only open vessels can be used
However, open containers are prone to contamination such as splashing, dripping, and aerosols during mixing and suction and discharge.
Especially in molecular microbiological testing, the viral load of strong positive samples is as high as 10 7 -10 9 Above, a slight aerosol generated during the operation may cause pollution and cause false positives
There is therefore always a risk of contamination in open containers
[0003] At the same time, due to the large workload of suction and discharge of liquid for many times, the structure of the automation equipment is complicated, and it is difficult to realize the miniaturization of the automation equipment.
[0004] Methods and devices that do not use conventional magnetic separation technology have not been generally recognized and accepted due to factors such as effects and costs, and their applicability is not strong

Method used

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  • Totally enclosed type magnetic separation system
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  • Totally enclosed type magnetic separation system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Such as figure 1 As shown, this embodiment provides a mixing device, which includes: a cavity 1 , a sample injection controller 3 , a sample outlet controller 4 , a magnet 5 and a mixer 6 .

[0055] Wherein, the shell of the cavity 1 is made of hard material, which is used to provide an operating space for mixing one or more sample liquids and magnetic beads. The sample liquid is various liquids such as a target solution containing a target substance and a solution containing surface-coated magnetic beads. The surface-coated magnetic beads are magnetic beads whose surface is coated with an adsorption substance for specific adsorption with a target substance. The average particle size of the magnetic beads is in the range of 0.01-100 microns. The cavity 1 is generally a combination of a cylindrical shape and a conical shape, but it can also be in other shapes.

[0056] The sample injection controller 3 is used to control the entry of the sample liquid, and has two ope...

Embodiment 2

[0077] The sample inlet controller 3 and the sample outlet controller 4 of the mixing device in the first embodiment are separated, however, they can also be combined together. Such as figure 2 As shown, this embodiment provides a mixing device, which includes: a cavity 11 , a sample inlet and outlet controller 13 , a magnet 15 and a mixer 16 .

[0078] Wherein, the cavity 11 provides an operating space for mixing one or more sample liquids with the magnetic beads, and its shell is made of hard material. The sample liquid is various liquids such as a target solution containing a target substance and a solution containing surface-coated magnetic beads. The surface-coated magnetic beads are magnetic beads whose surface is coated with an adsorption substance for specific adsorption with a target substance. The average particle size of the magnetic beads is in the range of 0.01-100 microns. The cavity 11 is generally a combination of a cylindrical shape and a conical shape, bu...

Embodiment 3

[0099] This embodiment provides a magnetic separation system, which includes: a cavity 21, a magnet 25, three sample injectors, a sample injection controller 23, a sample discharge controller 24, a mixer 26, a recovery device 29, and a drive device 30. Collection device 28.

[0100] Wherein, the cavity 21 is used for temporarily storing the sample liquid and providing an operation space for mixing the sample liquid and the magnetic beads, and its shell is made of hard material. The sample liquid is various liquids such as a target solution containing a target substance and a solution containing surface-coated magnetic beads. The average particle size of the magnetic beads is in the range of 0.01-100 microns. The cavity 1 is cylindrical as a whole, but may also be in other shapes.

[0101] The magnet 25 is used to apply a magnetic field to a part of the cavity 21 to attract the magnetic beads to the inner wall of the cavity 21 .

[0102] There are three sample injectors, nam...

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Abstract

The invention provides a totally enclosed type magnetic separation system. The totally enclosed type magnetic separation system comprises a cavity, a sample inlet controller and / or a sample outlet controller and a magnet body; a shell of the cavity is made of a hard material; the sample inlet controller and / or the sample outlet controller are / is connected with the cavity through an elastic pipeline; and the magnet body applies a magnetic field, used for adsorbing magnetic beads to the inner wall of the cavity, to at least part of a region of the cavity. The elastic pipeline enables the cavityto have certain vibration amplitude, positioning is facilitated, local uniform mixing of a fixing device can be realized through small amplitude and high frequency, and the traditional magnetic separation method can be completed in a totally enclosed container. In addition,only the cavity is subjected to local mixing, and the whole separation system does not need mixing, so that the influence onother parts of the separation system by mixing is effectively avoided, and high integration is easily realized.

Description

technical field [0001] The invention belongs to the field of magnetic separation and relates to a fully enclosed magnetic separation system. Background technique [0002] In biological and medical research and applications, magnetic particles (such as magnetic beads) are often used for separation and purification. The method is to use a magnet to suck out the magnetic particles for transfer, or to suck the magnetic particles on the inner wall of the container to suck out the waste liquid to achieve The purpose of separation and purification. In addition to separation, there are multiple washing processes in practical applications, so magnets or pipettes are required to enter and exit the reaction vessel multiple times, so only open vessels can be used. However, open containers are prone to contamination such as splashing, dripping, and aerosols during mixing and suction and discharge. Especially in molecular microbiological testing, the viral load of strong positive sample...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N1/38G01N33/531B03C1/02C12N15/10
CPCB03C1/02C12N15/1013G01N1/34G01N1/38G01N33/531
Inventor 冷彦宁
Owner 冷彦宁
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