A kind of detection system of nucleoside triphosphate compound and its application
A nucleoside triphosphate compound technology, applied in the detection system of nucleoside triphosphate compounds and its application fields, can solve the problems of high sensitivity and expensive instruments, and achieve the effect of simple equipment and easy popularization and application
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Embodiment 1
[0061] Embodiment 1 has 1 o 2 The ability to generate positively charged carbon dot solutions with 1 o 2 Response of the mixed system of capture agent 9,10-diphenylanthracene-2-boronic acid (DABA) mother liquor to ATP
[0062] (1) In the experimental group, add 45 μL of 1 mg / mL DABA mother solution and 67 μL of 1 mg / mL ATP mother solution to 2 mL of 0.02 mg / mL carbon dot aqueous solution. the absorption spectrum. A xenon lamp was selected as the excitation light source with a power of 0.8mV / cm 2 , light for 5min, and detect the absorption change of DABA in the range of 350-430nm every 30s, get figure 1 .
[0063] (2) In the control group, 45 μL of 1 mg / mL DABA mother solution was added to 2 mL of 0.02 mg / mL carbon dot aqueous solution, and after mixing evenly, the background absorption of the carbon dot mother solution was deducted to detect the absorption spectrum of DABA at this time. A xenon lamp was selected as the excitation light source with a power of 0.8mV / cm 2...
Embodiment 2
[0066] Embodiment 2 has 1 o 2 The ability to generate carbon dot solutions with 1 o 2 The Response of the Mixed System of Capture Agent 2-Anthracene Borate (ABA) Mother Liquor to GTP
[0067] (1) In the experimental group, add 20 μL of 0.5 mg / mL ABA mother solution and 44 μL of 1 mg / mL GTP mother solution to 2 mL of 0.01 mg / mL carbon dot aqueous solution. Absorption spectrum of ABA. A xenon lamp was selected as the excitation light source with a power of 0.8mV / cm 2, illuminated for 10min, and detect the absorption change of ABA in the range of 350-430nm every 20s.
[0068] (2) In the control group, add 20 μL of 0.5 mg / mL DABA mother solution to 2 mL of 0.01 mg / mL carbon dot aqueous solution, mix well, subtract the background absorption of the carbon dot mother solution, and detect the absorption spectrum of ABA at this time. A xenon lamp was selected as the excitation light source with a power of 0.8mV / cm 2 , illuminated for 10min, and detect the absorption change of AB...
Embodiment 3
[0071] Embodiment 3, has 1 o 2 The ability to generate carbon dot solutions with 1 o 2 Response of Mixed System of Capture Agent 9,10-Dinaphthyl-2-Anthracene Borate (NABA) Mother Liquor to UTP
[0072] (1) In the experimental group, add 36 μL of 4 mg / mL NABA mother solution and 88 μL of 1 mg / mL UTP mother solution to 2 mL of 0.04 mg / mL carbon dot aqueous solution. the absorption spectrum. A xenon lamp was selected as the excitation light source with a power of 0.8mV / cm 2 , illuminated for 5min, and detect the absorption change of NABA in the range of 350-430nm every 30s.
[0073] (2) In the control group, 36 μL of 4 mg / mL NABA mother solution was added to 2 mL of 0.04 mg / mL carbon dot aqueous solution, and after mixing evenly, the background absorption of the carbon dot mother solution was deducted to detect the absorption spectrum of NABA at this time. A xenon lamp was selected as the excitation light source with a power of 0.8mV / cm 2 , illuminated for 5min, and detect...
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