Quality detection method for Dendrobium devonianum Paxton

A quality inspection method and technology of Dendrobium denticola, which are applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as the inability to fully reflect the overall curative effect, achieve strong interpretation pertinence and applicability, accurate quality evaluation, and solve dimensionality. effects of disaster

Active Publication Date: 2018-08-14
北京蓝标一成科技有限公司
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  • Claims
  • Application Information

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Problems solved by technology

In traditional Chinese medicine under the guidance of traditional Chinese medicine theory, any...

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  • Quality detection method for Dendrobium devonianum Paxton
  • Quality detection method for Dendrobium devonianum Paxton
  • Quality detection method for Dendrobium devonianum Paxton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The generation sequencing of embodiment 1 dendrobium dendrobium

[0053] First-generation sequencing primer sequences:

[0054] ITS-26SE: 5'GAATTCCCCGGTTCGCTCGCCGTTAC 3';

[0055] ITS-17SE: 5' ACGAATTCATGGTCCGGTGAAGTGTTCG 3'.

[0056] The amplification and sequencing parameters are: denaturation at 98°C for 2 minutes and then PCR cycle, the PCR cycle parameters are 98°C for 20s; 52°C for 30s; 68°C for 1min, 38 cycles, 68°C for 7min, set 4°C after the amplification, and perform Generation Molecular Sequencing.

[0057] Through the first-generation sequencing, the species of Dendrobium to be tested was identified as Dendrobium dendrobii.

Embodiment 2

[0058] The extraction method of embodiment 2 dendrobium dendrobium

[0059] Take the dried sample of Dendrobium dendrobii, pulverize it with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder (weighing error cannot exceed 0.2%), place it in a 100ml Erlenmeyer flask, add 50mL 75 % methanol (V water: V methanol = 25:75), take it out after ultrasonication for 30 minutes at room temperature, filter, and concentrate the filtrate to dryness by rotary evaporation, dissolve it with 75% methanol solvent (V water: V methanol = 25:75), and finally transfer Dilute it to a 10ml volumetric flask, shake well, and filter with a 0.45 μm microporous membrane to obtain a Dendrobium dendrobium sample solution.

Embodiment 3

[0060] The chromatographic detection method of the Dendrobium dendrobium extract of embodiment 3

[0061] ①Preparation of reference solution

[0062] Accurately weigh 4.10 mg of Schaftoside and 4.08 mg of naringenin, place them in 10 ml volumetric flasks, add 75% (V / V) methanol to dissolve and dilute, shake well, and use them as stock solutions. Refrigerate at 4°C for later use.

[0063] Then accurately draw a certain amount of reference substance stock solution and dilute with 75% methanol to accurately prepare a mixed reference substance solution of schaffertoside and naringenin. Through different dilution ratios, 7 concentration points of the prepared components were diluted. into a high performance liquid chromatograph.

[0064] ②Extraction and treatment of samples for the determination of small molecule components in Dendrobium dendrobii:

[0065] Take 1.00g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, put it in a 100ml volumetric...

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Abstract

The invention discloses a quality detection method for Dendrobium devonianum Paxton. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the variety of a to-be-detected Dendrobium devonianum Paxton herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and/ornaringenin as a reference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium devonianum Paxton; and 4) establishing an analysis model by using the content value of the schaftoside and/or naringenin in the chromatographic data as the response variable and using the peak area value of other components in the fingerprint as the independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component. According to the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium devonianum Paxton is precisely identified and controlled.

Description

technical field [0001] The invention belongs to the field of detection and analysis of traditional Chinese medicine components, and in particular relates to a quality detection method of Dendrobium dendrobii. Background technique [0002] Dendrobium dendrobium (scientific name: Dendrobium devonianum Paxton): the stem is drooping, slightly fleshy, thin cylindrical, 50cm-70cm long, 0.3cm-0.5cm in diameter, unbranched, with multiple nodes, internodes 2.5cm-4cm long, stem Often light brown with black after. Leaf bases with clasping sheaths, leaf sheaths often with purple spots, papery after drying. Constant racemes, growing on the old stems of deciduous leaves, each inflorescence has 1-2 flowers; inflorescence stalk is green, about 0.4cm long, with 2-3 dry filmy sheaths at the base; flower bracts are membranous Quality, ovate, about 0.4cm long, apex nearly sharp; pedicel and ovary greenish brown, 2cm-2.5cm long; flower texture thin, spreading, with fragrance; middle sepal whit...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8686
Inventor 赵田邹婷婷
Owner 北京蓝标一成科技有限公司
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