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Polypeptide sequence, kit and method for detection of anti-interleukin-2 receptor subunit alpha natural antibody

A technology for detecting reagents and natural antibodies, applied in the field of immunology

Active Publication Date: 2018-08-21
杭州英邈生物科技有限公司
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Problems solved by technology

It is speculated that the risk of developing tumors in those who lack or are negative for natural antibodies may be significantly higher than that in the normal population

Method used

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  • Polypeptide sequence, kit and method for detection of anti-interleukin-2 receptor subunit alpha natural antibody
  • Polypeptide sequence, kit and method for detection of anti-interleukin-2 receptor subunit alpha natural antibody
  • Polypeptide sequence, kit and method for detection of anti-interleukin-2 receptor subunit alpha natural antibody

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Experimental program
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Embodiment Construction

[0066] 1. Sample collection

[0067] The plasma samples of 23 patients with early non-small cell lung cancer (stage I squamous lung cancer and glandular lung cancer) before surgery and 199 healthy people were collected for detection of plasma AIL2RA natural antibody. All plasma samples were stored at minus 80 degrees (-80°C) before operation. It was verified that the storage time did not exceed two years, and there was no repeated freezing and thawing (no more than 3 times).

[0068] 2. Sample detection

[0069] The plasma samples were thawed at 4°C. The three polypeptide antigens (see Table 2) used in this experiment were synthesized by chemical solid phase with a purity of 95%. The specific steps were as follows:

[0070] (1) Before operation, each antigen was dissolved in 67% acetic acid into a 5.7 mg / ml stock solution, then mixed in equal volumes and stored in a -20°C refrigerator.

[0071](2) At the beginning of the operation, at first the three kinds of antigens were d...

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Abstract

The invention relates to an antigen polypeptide for detection of an anti-interleukin-2 receptor subunit alpha (AIL2RA) natural antibody, a kit containing the antigen polypeptide, a method for detecting the concentration of AIL2RA natural antibody in human plasma with the antigen polypeptide or kit, and application of the antigen polypeptide or kit in detection of human plasma AIL2RA natural antibody. The invention utilizes 3 linear antigen polypeptides completely complementary to the target AIL2RA natural antibody to realize qualitative and quantitative detection of AIL2RA in plasma respectively, and the linear antigen polypeptides can be used for distinguishing plasma (positive) rich in the AIL2RA natural antibody and plasma (negative) without the AIL2RA natural antibody.

Description

technical field [0001] The invention belongs to the technical field of immunology and relates to polypeptide antigens of three amino acid sequences, which can be applied to the preparation of an enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA) antibody detection kit for detecting anti-interleukin-2 in plasma Receptor subunit A (Anti-interleukin-2 receptor subunit alpha, AIL2RA) natural antibody concentration. Background technique [0002] Malignant tumors are one of the most important killers threatening the health of the whole human being. Due to the continuous existence of environmental pollution such as smog and smog, the incidence of major cancers in the Chinese population is increasing year by year. According to statistics, the average annual incidence of cancer in my country is as high as 3 / 1000 or more. Early tumor treatment methods, such as surgical resection or radiation therapy, have been developed relatively maturely, which can signif...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 尉军张萱徐宏海
Owner 杭州英邈生物科技有限公司
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