Rotating culture device and culture vessel for use in rotating culture device
A technology of rotating culture device and incubator, which is used in tissue cell/virus culture devices, bioreactors/fermenters for specific purposes, tissue culture, etc. Complex structure, increased cost of large-scale and other problems, to achieve the effect of easy capacity, increased capacity, high processing accuracy
Inactive Publication Date: 2018-08-21
JTEC
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Problems solved by technology
However, its device structure is a complex structure with two axes, and the channel structure of the culture solution is also complicated, requiring a large amount of culture medium. Therefore, due to the obvious increase in the cost of large-scale, it is not a realistic choice. The removal of the incubator and the replacement of the culture medium There are also problems in operability and maintenance such as installation and installation, and it is difficult to grasp and control the dispersion of cell blocks
Method used
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Embodiment Construction
[0064] Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.
[0065] Such as Figure 1 ~ Figure 3 As shown, the rotary culture device 1 of the present invention includes: a cylindrical incubator 10 that is axially longer; a support mechanism 11 that supports the incubator 10 at both ends so that the incubator 10 can rotate around the axis; The rotary drive part 12 that drives the incubator 10 in rotation; the tilting motion part 13 that makes the support mechanism 11 tilt and move, so that the axis of the incubator 10 tilts up and down; A reciprocating part 14 that reciprocates integrally in the horizontal direction of the axis; an illuminating device 17 that illuminates the inside of the incubator 10 through an observation window 10a provided on the peripheral wall portion 20 of the incubator 10; 20 relative to the outer position, and the internal imaging unit 16 is photographed through the observation window 10a; acco...
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To provide, at low cost, a rotating culture device with which it is easy to understand or control a dispersed state of cell masses without worsening the ability to perform maintenance or conduct worksuch as removing a culture vessel, replacing a culture medium, installing a culture vessel, etc., and with which, even in a culture vessel having a large volume, cell masses can be cultured in a dispersed state without being locally concentrated. As a result, cell masses are prevented from colliding with each other, enabling large-scale culturing of high-quality uniform cell tissues and culturingof larger cells. The present invention is provided with a cylindrical culture vessel 10 which is axially elongated, a bearing mechanism 11 for bearing the culture vessel 10 so that the culture vessel10 can rotate about an axis, a rotational drive means 12 for driving the culture vessel 10 to rotate, a tilting means 13 for tilting the bearing mechanism 11 so that the axis of the culture vessel 10is inclined upward and downward, and a control means 15 for controlling operation of the rotational drive means 12 and the tilting means 13.
Description
technical field [0001] The present invention relates to a rotary culture device and an incubator used in the rotary culture device. More specifically, the present invention relates to a rotary culture device and a culture device suitable for mass culture of cells and large tissue culture. Background technique [0002] Mesenchymal stem cells derived from bone marrow have the ability to differentiate into tissues such as bone, cartilage, fat, and ligaments. It can be made into a two-dimensional cell membrane without losing the original cell shape characteristics. Therefore, various rotating culture devices have been proposed in recent years to realize three-dimensional culture in a microgravity environment close to zero gravity. [0003] For example, in Patent Documents 1 to 3, a device is proposed in which a flat cylindrical incubator with a gas permeable membrane disposed on the back side (rear side) is mounted on the horizontal rotation axis of the rotation control device ...
Claims
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IPC IPC(8): C12M1/10C12M1/00C12M3/00C12N5/02
CPCC12M27/10C12M3/043C12M23/06C12M23/22C12M23/24C12M23/48C12M23/50
Inventor 津村尚史小野贵弘
Owner JTEC