Preparation method of null type HMW-GS (High Molecular Weight Glutenin Subunits) wheat

Abstract

The invention discloses a preparation method of null type HMW-GS (High Molecular Weight Glutenin Subunits) wheat. The method comprises the following steps: taking tetraploid wheat for expressing nullglutelin as a female parent, taking diploid aegilops tauschii for expressing non-null glutelin as a male parent, carrying out non-homoploidy hybridization to obtain triploid hybrid F1, and enabling chromosome to be automatically reduplicated into hexaploid; then taking the hexaploid as a female parent, taking Glu-D1 wheat for expressing the null glutelin as a male parent, carrying out homoploidy hybridization to generate F1 and selfing to obtain F2, planting seeds for identifying expression of the null glutelin in fields and selfing to obtain F3, selfing to obtain F6 through a single seed descent method of the seeds, and planting the seeds to obtain a plant line F7, thus obtaining a wheat line stably expressing 0 glutelin. Indexes including sedimentation value, the content of wet gluten, the gluten index, the forming time and the stabilizing time of the line are lower than those of weak-gluten wheat Chuannong 16, so that the line has the effect of reducing the related indexes of the wheat gluten.

Description

technical field [0001] The invention belongs to the technical field of wheat variety breeding, and in particular relates to a method for creating null type HMW-GS wheat. Background technique [0002] Wheat can be processed into hundreds of foods because of its "gluten protein" that is not found in other cereal crops. Wheat "gluten protein" includes three categories: high molecular weight glutenin subunits (HMW-GS), low molecular weight glutenin subunits (LMW-GS) and gliadin. Wheat HMW-GS accounts for about 10% of the total protein in mature seeds, but it acts as a "skeleton" in the gluten network, and its HMW-GS composition and expression number can affect gluten strength and ultimately determine the processing quality of wheat flour (Shewry and Halford 2002 ; Shewry et al. 2003; Yang et al. 2014; Wieser and Kieffer; 2001). [0003] There is a compound gene locus encoding HMW-GS on the long arm of the first homologous group chromosome of common wheat, collectively referred...

AI Technical Summary

Problems solved by technology

There are few varieties of weak-gluten wheat, poor stability of quality indicators, and difficulty in flour blending are the main problems faced by the production of weak-gluten wheat in my country

[0005] To sum up, the existing problems in the prior art are: although reducing the number of HMW-GS expressed in the background of common wheat can reduce the processing quality indicators of wheat flour related to gluten strength to a certain extent, and become a phenomenon that the characteristics of cultivated gluten tend to be weakened. Potential new avenues for breeding wheat varieties

However, the number of HMW-GS expressed in natural hexaploid wheat resources (including bred varieties) is too much, usually 3-5, rarely less than 3, and no expression of 0 HMW-GS can be found. GS (i.e. not expressing any HMW-GS protein) hexaploid wheat

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