Method for improving flavor of high-salt liquid fermentation soy sauce with various salt-tolerant yeasts
A technology of high-salt dilute fermentation and salt-tolerant yeast, which is applied in the field of food processing, can solve the problems of undiscovered patent documents, etc., and achieve the effect of increasing the variety of flavor substances, rich nutrition, and easy operation
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[0041] (1) Preparation of seed koji: use bran as raw material, mix water according to the weight ratio of bran: water of 1:1.1, stir well, put it into a conical flask, and then sterilize at 121°C for 20 minutes to obtain seed koji culture The three-to-four-ring Aspergillus oryzae spores were picked from the PDA medium, inoculated into the sterilized seed koji medium in a sterile environment, mixed well, stacked and cultivated in a 32°C constant temperature incubator, and cultivated for 14 After ~16 hours, shake the flask for the first time, break up the koji materials, so as to achieve the purpose of secondary inoculation, then adjust the temperature to 30°C for flat culture, and shake the flask for the second time after 6~8 hours, and cultivate for 70~ After 74 hours, the seed koji is mature, put it into a bag and dry it, and get the seed koji for later use;
[0042] (2) Soaking: The mass ratio of soybean meal: fried wheat is 6:4, and the amount of mixing water is 100% to 110...
Embodiment 1
[0057] A method for improving the flavor of high-salt dilute fermented soy sauce by adding a variety of salt-tolerant yeasts, the method is by adding different combinations of salt-tolerant yeasts to high-salt dilute fermented soy sauce at different times, thereby improving the quality of soy sauce products Improve, the flavor is improved;
[0058] Specifically, the steps are as follows:
[0059] (1) Preparation of seed koji: use bran as raw material, mix water according to the weight ratio of bran: water of 1:1.1, stir well, put it into an Erlenmeyer flask, and then sterilize it at 121°C for 20min to obtain the seed Aspergillus oryzae spores of three to four rings were picked from the PDA medium, inoculated into the sterilized koji medium in a sterile environment, mixed well, and stacked in a constant temperature incubator at 32°C. After 14-16 hours of cultivation, shake the flask for the first time, break up the koji material, so as to achieve the purpose of secondary inocu...
Embodiment 2
[0067] A method for improving the flavor of high-salt dilute fermented soy sauce by adding a variety of salt-tolerant yeasts, the method is by adding different combinations of salt-tolerant yeasts to high-salt dilute fermented soy sauce at different times, thereby improving the quality of soy sauce products Improve, the flavor is improved;
[0068] Specifically, the steps are as follows:
[0069] (1) Preparation of seed koji: use bran as raw material, mix water according to the weight ratio of bran: water of 1:1.1, stir well, put it into an Erlenmeyer flask, and then sterilize it at 121°C for 20min to obtain the seed Aspergillus oryzae spores of three to four rings were picked from the PDA medium, inoculated into the sterilized koji medium in a sterile environment, mixed well, and stacked in a constant temperature incubator at 32°C. After 14-16 hours of cultivation, shake the flask for the first time, break up the koji material, so as to achieve the purpose of secondary inocu...
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