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Method for alleviating freezing damage to pear fruitlets

A technology for young pear fruit and freezing damage, applied in the field of antifreeze agent for young pear fruit, can solve the problem of less freezing damage of young pear fruit, and achieve the effect of reducing unexpected economic losses

Pending Publication Date: 2018-08-28
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Over the years, many scholars at home and abroad have done some research on freezing damage to fruit trees, but there are relatively few studies on freezing damage to young pears

Method used

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  • Method for alleviating freezing damage to pear fruitlets
  • Method for alleviating freezing damage to pear fruitlets
  • Method for alleviating freezing damage to pear fruitlets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment one: the impact of frost time on young fruit membrane lipid peroxidation

[0033] (1) Experimental process

[0034] After 10 days of pollination of "Cuiyu" pear young fruit, the artificial climate chamber was used to simulate the natural frost process: the temperature was lowered from 18°C ​​at a rate of 4°C / hour, and maintained at -2°C at 0, 1, 2, 3, 4 and 5 After one hour, young fruits with the same size and different positions were taken at different time points to measure the content of malondialdehyde.

[0035] (2) Experimental results

[0036] Flower and fruit drop caused by frost is mostly caused by the destruction of flower and young fruit cell membranes, and active oxygen free radicals play an important role in this process. The change of malondialdehyde content in young fruit under low temperature stress can directly reflect the degree of oxidation of cell membrane lipid and the permeability of cell membrane. At the beginning of freezing, the con...

Embodiment 2

[0038] Embodiment two: the screening of antifreeze

[0039] (1) Experimental process

[0040] Spray 500mL of 10 kinds of chemical antifreeze on the young fruit 10 days after pollination, take the water treatment as the control, and put it into the artificial climate chamber for low temperature treatment after 2 days. The young fruits with the same size and different positions were taken to determine the malondialdehyde content.

[0041] Determination of malondialdehyde content: Thiobarbituric acid colorimetric method. Take 0.50g young fruit sample, add 5mL of 5% (g / 100ml) trichloroacetic acid, and centrifuge the resulting slurry at 3000r / min for 10min after grinding. Take 2ml of the supernatant, add 2mL of 0.67% (g / 100ml) thiobarbituric acid, mix it in a water bath at 100°C for 30min, cool and then centrifuge. The absorbance values ​​at 450nm, 532nm and 600nm were measured respectively.

[0042] The 10 kinds of chemical antifreeze agents are Bibo PBO, Tianda 2116, prohexad...

Embodiment 3

[0050] Example 3: Verification and combination of the screened antifreeze agents

[0051] (1) Experimental process

[0052] Spray 500mL of the antifreeze selected in Example 2 individually / combinedly. The combined antifreeze is prepared from 6-benzylaminoadenine and salicylic acid, the concentration of 6-benzylaminoadenine in the aqueous solution is 0.50mg / L, and the concentration of salicylic acid is 3.62mmol / L. Taking the water treatment as the control, put it into the artificial climate chamber for low temperature treatment after 2 days. The young fruits with the same size and different positions were taken to determine the malondialdehyde content.

[0053] (2) Experimental results

[0054] The study found that 6-benzylaminoadenine, salicylic acid and combined antifreeze can significantly inhibit the freezing damage of young fruit; among them, the combination of antifreeze has a better effect (attached Figure 5 ). After freezing treatment, the malondialdehyde content ...

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Abstract

The invention discloses a method for alleviating the freezing damage to pear fruitlets. According to the invention, an antifreezing agent aqueous solution is sprayed before frost, wherein an antifreezing agent is any one or a combination selected from the group consisting of 6-benzylaminoadenine and salicylic acid. The method provided by the invention selects the antifreezing agent prepared from the 6-benzylaminoadenine and the salicylic acid, wherein the concentration of 6-benzylaminoadenine in an aqueous solution is 0.30 to 0.70 mg / L, and the concentration of the salicylic acid is 3.0 to 4.2mmol / L. The antifreezing agent is sprayed in 2 days before the frost, so the freezing damage to the pear fruitlets can be significantly alleviated. The antifreezing agent selected by the method provided by the invention can provide a theoretical basis and a practical guidance for prevention of late frost freezing damage to the pear fruitlets, and reduces accidental economic loss of fruit farmersdue to frost-freezing which frequently occurs before and after a pear flowering period.

Description

technical field [0001] The invention belongs to the technical field of antifreeze agents for young pear fruits, and in particular relates to a method for reducing freezing damage of young pear fruits. Background technique [0002] Frost, also known as freezing injury, refers to the damage caused by the freezing of plant tissues at a low temperature of 0°C or below. my country has a vast territory and a wide area of ​​crop cultivation, and the average annual frost damage area is 340,000 km 2 , the worst year reached 770,000 km 2 , causing at least 3 billion yuan in losses. In recent years, late frost damage has been particularly severe in northern my country. Some fruit trees that bloom early in spring often encounter late frost damage. The light ones reduce production, and the severe ones fail to harvest. [0003] There are many types of frost. According to the season of frost occurrence, it is divided into spring frost (commonly known as "falling spring cold"), autumn f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00A01N43/90A01N37/40A01P21/00
CPCA01G13/00A01N37/40A01N43/90
Inventor 张绍铃王利斌周玉莲谢智华齐开杰
Owner NANJING AGRICULTURAL UNIVERSITY
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