Induction method for lilium lancifolium callus tissue
A technology of callus and lily lily, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problem that it is difficult to induce the pollution rate of explant callus of lily lily, and achieve the solution of high pollution rate and Difficult to produce callus technical problems, easy to obtain materials, rapid propagation effect
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Embodiment 1
[0038] 1) Pretreatment of Lily Lilium bulbils
[0039] Clean the dirt on the surface of the Lily Lily bulbils under tap water, pick out the bulbils with mechanical damage, diseases and insect pests on the surface, soak the healthy bulbils in soapy water for 7-8 minutes, and then rinse them with running tap water for more than 2 hours.
[0040] 2) sterilization of lily bulbils
[0041] Divide the bulbils with a dissecting needle on the aseptic operating table and divide them into several uniform-sized bulbil scales, first clean and disinfect with 75% ethanol for 30 seconds, wash with high-temperature sterilized water for 2-3 times, and then add 0.20% mercuric chloride solution (mercuric chloride solution should submerge the bulbil scales), cover the container lid and shake it on a shaker for 10-15 minutes, let the mercury chloride solution fully contact with the bulbil scales to make the disinfection more thorough, and then put the mercury chloride solution on the sterile opera...
Embodiment 2
[0050] 1) Pretreatment of Lily Lily scales
[0051] Wash off the soil and impurities on the surface of the lily bulbs under tap water, peel off the scales on the periphery of the bulbs that are affected by diseases and insect pests or have other damages, and take the middle layer without mechanical damage, no diseases and insect pests, and healthy scales, and use a certain concentration of Sapindus Soak in saponin solution for 5 minutes, then rinse with running tap water for more than 2 hours and set aside.
[0052] 2) Sterilization of Lily Lily scales
[0053] Use 70% ethanol to sterilize the cleaned scales for 30 seconds on the aseptic operating table, and then wash them with high-temperature sterilized water for 2-3 times, then add 0.10% mercury liter solution to soak, cover the container, and shake it on a shaker for 20 seconds. -24min, make the scale fully contact with the mercuric chloride solution, pour out the mercuric chloride solution on the aseptic operating table ...
Embodiment 3
[0061] 1) Pretreatment of Lily Lily Bulb Disc
[0062] After washing off the soil and impurities on the surface of the lilac balls under tap water, cut the bulb disc from the base of the bulb with a knife, take a healthy base disc that is free from diseases and insect pests, mechanically damaged, soak it in soapy water for 10 minutes, and then wash it with clean water Let wash under clean flowing water for more than 3 hours.
[0063] 2) Sterilization of Lily Lily Bulb Disc
[0064] Disinfect the cleaned bulb disc with 75% ethanol for 1 min on the aseptic operating table, wash it with high-temperature sterilized water for 2-3 times, then soak the bulb disc with 0.20% mercuric solution for 10-15 min, and place it on a shaker during the period. It keeps shaking, and its purpose is to allow the bulb base plate to be fully soaked by the mercuric chloride solution to make the disinfection more thorough. Finally, pour out the disinfectant and add high-temperature sterilizing water t...
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