A kind of preparation method of peat moss propagule for peatland restoration
A technology of peat moss and propagules, which is applied in the field of preparation of peat moss propagules for peatland restoration, can solve the problems affecting the temperature and humidity of the peatland to be restored, achieve strong adaptability, improve survival rate, and have little impact on the ecological environment Effect
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Embodiment 1
[0024] Collect peat moss in the field, sort and clean the capsules, soak and disinfect the capsules in 75% alcohol for 3 minutes, rinse them with sterile water, and break them in the medium according to the ratio of adding 5 mL of liquid medium to each capsule The spore capsule is prepared to prepare a spore suspension to obtain a sexual propagule suspension of Sphagnum moss. Add 2% (w / v) sodium alginate solution to the suspension and stir it into a uniform viscous liquid, add the mixed suspension dropwise into 0.1M calcium chloride solution and harden it for 30 minutes to complete the sexual propagule of Sphagnum moss Preparation of Gel Particles. Sprinkle the prepared sphagnum sexual propagule gel particles into the peatland to be restored at a density of 500 per square meter, and after they are colonized and grown, the vegetation reintroduction process required for peatland restoration can be realized.
Embodiment 2
[0026] Collect sphagnum moss in the field, after sorting and cleaning, cut the fresh plants into 2cm-long fragments, soak the plant fragments in 1% sodium hypochlorite solution for 1min, rinse with sterile water and add them to liquid medium to make suspension liquid, that is, to obtain the sphagnum moss clonal propagule suspension. Add 5% (w / v) sodium alginate solution to the suspension and stir it into a uniform viscous liquid, add the mixed suspension dropwise into 0.1M calcium chloride solution and harden it for 60 minutes to complete the sexual propagule of Sphagnum moss Preparation of Gel Particles. Sprinkle the prepared sphagnum sexual propagule gel particles into the peatland to be restored at a density of 2000 per square meter, and after they are colonized and grown, the vegetation reintroduction process required for peatland restoration can be realized.
Embodiment 3
[0028] Collect sphagnum moss in the field, after sorting and cleaning, take the capsules and cut the fresh plants into 2cm long fragments, soak the capsules in 75% alcohol for 3 minutes, rinse them with sterile water, and divide them according to each capsule Add 5mL of liquid medium to break the spore capsules in the medium to prepare a spore suspension, soak the plant fragments in 1% sodium hypochlorite solution for disinfection for 1min, rinse with sterile water and add to the spore suspension to obtain Sphagnum moss Synthetic propagule suspension. Add 3% (w / v) sodium alginate solution to the suspension and stir to form a uniform viscous liquid. Add the mixed suspension dropwise to 0.1M calcium chloride solution and harden it for 45 minutes to complete the peat moss propagule gel Preparation of Granules. Sprinkle the prepared peat moss sexual propagule gel particles into the peatland to be restored at a density of 1000 per square meter, and after they are colonized and gro...
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