Method of determining relationships of gene expression and methylation modification regulation of predetermined species
A technology of gene expression and methylation, applied in the field of epigenetic regulation and molecular genetics, can solve the problem that the relationship between gene expression methylation modification and regulation of predetermined species has not been determined, and achieve high accuracy and good repeatability Effect
Active Publication Date: 2018-09-07
BEIJING FORESTRY UNIVERSITY
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[0003] However, so far, there are no reports identifying the relationship between gene expression and methylation modification regulation in a predetermined species
Method used
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Embodiment 1
[0061]With reference to the method for determining the relationship between gene expression and methylation modification regulation of a predetermined species of the present invention, according to the steps of the above-mentioned general method, determine the relationship between gene expression and methylation modification regulation of Populus tomentosa, specifically as follows:
[0062] Among them, in this example, Populus tomentosa L9 is used as the male parent of hybridization, and LM50 is used as the female parent.
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The invention discloses a method of determining relationships of gene expression and methylation modification regulation of a predetermined species. The method comprises: (1) using a male parent and afemale parent of the predetermined species and a progeny sample thereof as to-be-tested samples, and carrying out bisulfite whole-genome methylation sequencing; (2) determining all cytosine sites inthe progeny sample and reads comprising the cytosine sites; (3) determining reads which are in the reads comprising the cytosine sites and and belong to allele sequences; (4) determining an apparent genotype of each pair of target reads; (5) carrying out gene classification on the target fragments; (6) carrying out counting of the number of target fragments comprised by each gene and the ratio ofthree apparent genotypes, and obtaining expression level information of each gene; (7) obtaining various candidate gene combinations by division; (8) carrying out Pearson correlation analysis; (9) screening out a target gene combination; and (10) carrying out multiple linear regression. Therefore, the relationships of gene expression and methylation modification regulation of the predetermined species can be effectively determined.
Description
technical field [0001] The present invention relates to the technical field of molecular genetics, specifically to the technical field of epigenetic regulation, and more specifically, to a method for determining the relationship between gene expression and methylation modification regulation of a predetermined species. Background technique [0002] Gene expression is regulated by multidimensional factors and is very complex. It is of great significance for the study of epigenetic regulation to separate the single regulatory factor from the multidimensional factor regulatory network, construct a mathematical model of its influence on gene expression, and then determine the influence of the single regulatory factor on gene expression regulation. Among them, DNA methylation is a very important epigenetic regulatory factor. Therefore, it is crucial to construct a mathematical model of how DNA methylation affects gene expression in a certain species, and to determine the relation...
Claims
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IPC IPC(8): G06F19/20
CPCG16B25/00
Inventor 张德强次东宋跃朋
Owner BEIJING FORESTRY UNIVERSITY
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