Preparation method of a pulp capping material in vitro evaluation model

Abstract

This patent application relates to the technical field of dental preparations, in particular to the technical field of in vitro evaluation of pulp capping materials. The invention discloses a method for preparing an in vitro evaluation model of a pulp capping material, which is characterized in that it comprises the following steps: S1. Obtaining an isolated tooth; S2. Isolating dental pulp cells; S3. Expanding cells; S4. Adding ECM to form spheres and grow ; S5. Continuous induction, preparing human dentine extract, adding a medium containing dentine extract to a culture plate to continuously induce cells, and making a human dental pulp organoid model. The present invention selects the isolated human dental pulp cells, undergoes cell separation and expansion, adopts a invasive cell growth method, and finally adopts a continuous induction method, the growth efficiency of the cells is high, and the human dental pulp organs are highly simulated. The efficiency and accuracy of the in vitro evaluation of the entire material are improved.

Description

technical field [0001] The invention belongs to the technical field of dental preparations, in particular to the technical field of in vitro evaluation of pulp capping materials. Background technique [0002] At present, every year in our country, a large number of patients have dental pulp exposed or nearly exposed due to caries or trauma. Infection can form lesions, causing or adding arthritis, pericarditis, and various eye diseases and other systemic diseases. At present, in view of the above situation, in the absence of obvious irreversible infection, the affected tooth needs to receive pulp capping treatment, that is, the pulp capping material is directly placed on the exposed pulp tissue to seal the exposed pulp, avoid infection and further stimulation, and promote The role of local dentin bridge formation. Pulp capping, also known as pulp capping, is a method of protecting the living pulp, that is, covering the dentin near the pulp or the exposed pulp wound with a p...

AI Technical Summary

Problems solved by technology

The current in vitro cell model is to inoculate human dental pulp cells on a culture plate, and the cells grow in a single layer, while the cells grow in a three-dimensional state in vivo. Using this model for drug screening and detection cannot reflect the actual situation in the human body

There are many options for animal in vivo models, such as rodents, canines, primates, etc., using rodents or canines, the dental pulp cells and human dental pulp tissue cells are very different, such as mouse Anterior teeth can grow all their lives, so this method can only reflect the toxicity or biocompatibility of drugs on dental pulp tissue to a certain extent, but there are differences from human dental pulp tissue; while using primates, it is costly Huge, and there is a possibility of violating the requirements of ethical review

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