Preparation method of a pulp capping material in vitro evaluation model

A technique for evaluating models and material bodies, applied in biochemical equipment and methods, cell dissociation methods, microorganisms, etc. Capacitance, more scientific evaluation results, and improved efficiency and accuracy
CN108517312BActive Publication Date: 2021-06-01STOMATOLOGICAL HOSPITAL OF CHONGQING MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
STOMATOLOGICAL HOSPITAL OF CHONGQING MEDICAL UNIV
Publication Date
2021-06-01

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Abstract

This patent application relates to the technical field of dental preparations, in particular to the technical field of in vitro evaluation of pulp capping materials. The invention discloses a method for preparing an in vitro evaluation model of a pulp capping material, which is characterized in that it comprises the following steps: S1. Obtaining an isolated tooth; S2. Isolating dental pulp cells; S3. Expanding cells; S4. Adding ECM to form spheres and grow ; S5. Continuous induction, preparing human dentine extract, adding a medium containing dentine extract to a culture plate to continuously induce cells, and making a human dental pulp organoid model. The present invention selects the isolated human dental pulp cells, undergoes cell separation and expansion, adopts a invasive cell growth method, and finally adopts a continuous induction method, the growth efficiency of the cells is high, and the human dental pulp organs are highly simulated. The efficiency and accuracy of the in vitro evaluation of the entire material are improved.
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Description

technical field

[0001] The invention belongs to the technical field of dental preparations, in particular to the technical field of in vitro evaluation of pulp capping materials. Background technique

[0002] At present, every year in our country, a large number of patients have dental pulp exposed or nearly exposed due to caries or trauma. Infection can form lesions, causing or adding arthritis, pericarditis, and various eye diseases and other systemic diseases. At present, in view of the above situation, in the absence of obvious irreversible infection, the affected tooth needs to receive pulp capping treatment, that is, the pulp capping material is directly placed on the exposed pulp tissue to seal the exposed pulp, avoid infection and further stimulation, and promote The role of local dentin bridge formation. Pulp capping, also known as pulp capping, is a method of protecting the living pulp, that is, covering the dentin near the pulp or the exposed pulp wound with a p...

Claims

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