Method for creating waterlogging-resistant wax gourd mutants by mutagenic agents EMS

A mutagen and mutant technology, which is applied in the field of creation of wax gourd germplasm resources, can solve problems such as lack of waterlogging-tolerant germplasm resources in wax gourd, and achieve the effects of remarkable mutagenesis effect, expansion of germplasm resources sources, and simple method.

Pending Publication Date: 2018-09-14
HUNAN VEGETABLE RES INST
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Problems solved by technology

[0005] The object of the present invention is to provide a kind of method that adopts EMS mutagen to create waterlogging-toleran...

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  • Method for creating waterlogging-resistant wax gourd mutants by mutagenic agents EMS
  • Method for creating waterlogging-resistant wax gourd mutants by mutagenic agents EMS
  • Method for creating waterlogging-resistant wax gourd mutants by mutagenic agents EMS

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Embodiment Construction

[0023] Embodiments of the invention are described in detail below, but the invention can be practiced in many different ways as defined and covered by the claims.

[0024] The invention provides a kind of method adopting EMS mutagen to create the waterlogging-tolerant mutant of wax gourd, comprising the following steps:

[0025] (1) Screening of optimal conditions for EMS mutagenesis

[0026] Before carrying out the EMS mutagen to create waterlogging-tolerant mutants of wax gourd, a comparative experiment was carried out on the conditions and methods of EMS mutagenesis. Regularity (it takes about 36 hours for the germination of wax gourd to start to show white, and it takes about 72 hours to germinate). After washing the seeds of wax gourd with clean water, they are placed in a warm soup at 55°C to soak the seeds for 30 minutes. 28 ℃ incubator germination 36h. Then, different EMS concentrations and time treatments were performed. 100 seeds were selected for each treatment. ...

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Abstract

The invention provides a method for creating waterlogging-resistant wax gourd mutants by mutagenic agents EMS. The method includes (1), treating wax gourd seeds by EMS phosphate buffer liquid with thevolume ratio of 1-1.3% for 10-15hours to obtain M1-generation seeds; (2), putting the M1-generation seeds treated by the mutagenic agents EMS into a CO2 constant-temperature pregermination box, withthe temperature of 25-30 DEG C and the oxygen concentration of 10-15%, for pregermination; (3), sowing the M1-generation seeds, acquiring M2-generation seeds after selfing, sowing the M2-generation seeds, performing selfing and pollination after 15-25 days, soaking the basal parts of stems by 15-25cm for 3-4 hours in a field, selecting waterlogging-resistant wax gourd plants, harvesting and reserving seeds of the waterlogging-resistant wax gourd plants per gourd to obtain the waterlogging-resistant wax gourd mutants Mut. The method has the advantages that the wax gourd seeds are mutagenized bythe chemical mutagenic agents EMS to obtain the waterlogging-resistant wax gourd mutants, so that sources of genetic resources are widened, and new genetic resources are created.

Description

technical field [0001] The invention relates to the technical field of creation of winter melon germplasm resources, in particular to a method for creating winter melon waterlogging-tolerant mutants by using EMS mutagen. Background technique [0002] Winter melon is the main vegetable crop in my country, with an annual planting area of ​​about 4.8 million mu. Winter melon products play an important role in regulating the annual supply of vegetables in my country. Because wax gourd is mainly distributed in Southeast Asian countries such as my country, India, Vietnam, Laos, etc. However, compared with other vegetable crops, the genetic background of wax gourd materials is relatively narrow, which seriously limits the creation of new materials of wax gourd and the expansion of high-quality resources. At present, winter melon lacks waterlogging-tolerant germplasm resources, and the main planting areas of winter melon are in the Yangtze River Basin and the south of the Yangtze Ri...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H1/06
CPCA01H1/04A01H1/06
Inventor 弭宝彬周火强谢玲玲武芳芳戴雄泽张竹青
Owner HUNAN VEGETABLE RES INST
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