Prostatic cancer high-fat diet model establishment method and application

A technology for prostate cancer and high-fat diet, applied in the fields of biomedicine and genetic engineering, can solve the problems of changes in circulating cytokines and the related mechanism of the impact of prostate cancer has not been fully elucidated, and achieves simple modeling operation, high success rate, and usefulness. wide range of effects

Inactive Publication Date: 2018-09-14
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the relationship between obesity and prostate cancer diagnosis, pathological outcome, and prognosis is not yet clear, especially in Asian populations; and the related mechanism of obesity and high-fat diet on prostate cancer has not been fully elucidated, especially high-fat diet mediation. induced changes in circulating cytokines in vivo

Method used

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  • Prostatic cancer high-fat diet model establishment method and application
  • Prostatic cancer high-fat diet model establishment method and application
  • Prostatic cancer high-fat diet model establishment method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1: Construction of TRAMP prostate cancer mouse model

[0032]Using TRAMP mice (transgenic adenocarcinoma of mouse prostate, TRAMP, purchased from the Jackson laboratory in the United States, raised and bred in the Institute of Development and Growth, Fudan University), the amputated toes were immediately tested for genes after birth, and the amputated toes were placed in the EP tube. Add 500ul lysate (50mM Tris, 100mM EDTA, 0.5% SDS) and 25ul proteinase K (10mg / ml) and mix well, incubator at 55d°C overnight; add 500ul phenol chloroform the next day, centrifuge at 13200 rpm for two minutes, Discard the precipitate, transfer 200ul supernatant to EP tube, add 200ul phenol chloroform to mix, centrifuge at 13200 rpm for 1 minute, discard the precipitate again, transfer 100ul supernatant to EP tube, add 250ul absolute alcohol and 3M acetic acid Mix 25 ul of sodium, centrifuge at 13200 rpm for 1 minute, discard the supernatant, wash the precipitate twice with 75% alcoh...

Embodiment 2

[0034] Example 2: Construction of TRAMP mouse high-fat diet model

[0035] After genotype identification, only male mice carrying the target gene were retained; the mice were separated into cages after weaning 20 days after birth, and were divided into the control group and the experimental group, and then fed with ordinary feed and high-fat feed respectively. The feed ingredients were shown in Table 1. Feed feeding started from the day of weaning to the day before killing and collecting the animals. The indoor temperature of the animals was maintained at 23°C±1°C, and the humidity was maintained at 60%±10%. , 3 times of litter, fasting and water for 10 hours before execution, at 20w, 24w, 28w, the mice were executed in 3 batches;

[0036] The basic situation, tumor formation, tumor differentiation, and tumor metastasis of TRAMP mice fed with common feed and high-fat feed (high-fat feed as shown in Table 1) are shown in Table 2. Both the TRAMP mice in the diet group and the T...

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Abstract

The invention belongs to fields of biomedicine and genetic engineering and relates to a TRAMP (transgenic adenocarcinoma of mouse prostate) mouse high-fat diet model establishment method and application in prostatic cancer prevention and treatment process research platforms. A prostatic cancer obesity model which takes 40% fat energy supply feed as an intervention measure on the basis of TRAMP mice. Prostate tissue tumor formation, differentiation and metastasis of the model mice are higher than those of TRAMP mice fed with common feed. By comprehensive means of physical measurement, blood biochemical examination, imageological examination and the like, stability of the prostatic cancer obesity model is assessed, and an objective, uniform, stable and precise model and experimental platformfor obesity related factors in prostate cancer occurrence and development are established. The model well reflects influences of high-fat diet on prostate cancer occurrence and development and has ahigh application value in fields of prostatic cancer mechanism research and corresponding medicine screening and efficacy assessment.

Description

technical field [0001] The invention belongs to the fields of biomedicine and genetic engineering, and relates to a method for establishing a high-fat diet model, in particular to a method for establishing a TRAMP mouse high-fat diet model and its application in the process of preventing and treating prostate cancer. Background technique [0002] Transgenic prostate cancer mouse (transgenic adenocarcinoma of mouse prostate, TRAMP) model is one of the most commonly used transgenic prostate cancer models. In 1995, Greenberg et al. first reported the establishment of this animal model. The regulated probasin promoter combined with Simian Virus 40 (SV40) T antigen was transferred into mice to specifically induce tumors in the mouse prostate. T antigen began to be expressed in 4-6 weeks, and the tumor protein produced by SV40 could block tumor suppressor genes The p53 and RB pathways, thereby leading to tumor formation, and pathological progression similar to human prostate cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A01K67/02A61K49/00
CPCA01K67/02A01K67/0278A01K2217/00A01K2227/105A01K2267/0331A01K2267/0362A61K49/0008C12Q1/6886
Inventor 姜昊文许华胡吉梦胡梦博杨天朱文辉刘宇飞
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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