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Tungsten disulfide nanosheet-based label-free biosensor, as well as preparation method and application thereof

A biosensor, tungsten disulfide technology, applied in the field of optical biosensors, can solve the problems of high cost of nucleic acid detection, low cost of nucleic acid, cumbersome operation, etc., and achieve the effect of reducing detection cost, high sensitivity, and simplifying operation steps

Active Publication Date: 2018-09-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the high cost and cumbersome operation of existing fluorescent sensors for detecting nucleic acids, the present invention provides a label-free optical sensor based on tungsten disulfide nanosheets. The cost of using the label-free optical sensor to detect nucleic acids is low and the operation is simple

Method used

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  • Tungsten disulfide nanosheet-based label-free biosensor, as well as preparation method and application thereof
  • Tungsten disulfide nanosheet-based label-free biosensor, as well as preparation method and application thereof
  • Tungsten disulfide nanosheet-based label-free biosensor, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] In this embodiment, the shear exfoliation method is used to exfoliate bulk tungsten disulfide into two-dimensional layered tungsten disulfide nanosheets. The specific method is as follows:

[0054] Weigh 50g of bulk tungsten disulfide powder and 5g of sodium cholate powder, mix the two, add 500mL of ultrapure water, mix well, put them into a household blender, and stir at a speed of 12000rpm. To prevent excessive foam generation, take intermittent stirring, that is, stir for three minutes, stop for three minutes, and the total stirring time is 120 minutes.

[0055] The stirred product was centrifuged at 1500 rpm for 90 minutes, and half of the supernatant of the centrifuged product was taken to obtain a sodium cholate solution suspension of tungsten disulfide nanosheets.

[0056] Since the obtained two-dimensional layered tungsten disulfide nanosheets are dispersed in the sodium cholate solution, there is a certain impact on the subsequent experiments, so the product n...

Embodiment 2

[0058] After adding NaCl to the tungsten disulfide nanosheet suspension prepared in Example 1, detect the change of its ultraviolet light absorption peak, the specific method is as follows:

[0059] Take 200 μL of the tungsten disulfide nanosheet suspension prepared in Example 1 in 1600 μL of ultrapure water, add 200 μL of NaCl solution with a concentration of 1M (mol / L), and the final concentrations of tungsten disulfide nanosheet and NaCl are respectively 0.05 mg / mL and 0.1M.

[0060] Measure the ultraviolet-visible absorption spectra of the suspension at 0, 1, 2, 4, 8, 16, and 20 minutes respectively, such as figure 1 shown.

[0061] It can be seen that the absorption peak of the tungsten disulfide nanosheet suspension moves from 625nm to 630nm, indicating that under the action of sodium ions, the dispersed tungsten disulfide nanosheets gather to increase the size and thickness of the sheet.

[0062] When the tungsten disulfide nanosheets gather to a certain extent, they...

Embodiment 3

[0067] Different concentrations of metal cations have different sedimentation effects on tungsten disulfide nanosheets. Therefore, it is necessary to optimize the ratio of tungsten disulfide nanosheets to metal cations. The specific method is as follows:

[0068] Take 200 μL of the tungsten disulfide nanosheet suspension prepared in Example 1, mix with different volumes of NaCl solution and ultrapure water, so that the total volume is 2mL, and the concentration of NaCl is respectively 0M, 0.001M, 0.025M, 0.005M, 0.01M, 0.25M, 0.05M, 0.1M.

[0069] After mixing evenly, let it stand, take two-thirds of the supernatant of each bottle at intervals of one hour, measure the ultraviolet-visible absorption spectrum of the supernatant, and divide the absorbance at 630nm of each concentration by the blank group (NaCl Concentration is 0) the absorbance at 630nm place, this value is set as ordinate, time is abscissa, the result is as follows image 3 As shown, choose 0.05M NaCl concentra...

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Abstract

The invention discloses a preparation method for a tungsten disulfide nanosheet-based label-free biosensor. The preparation method comprises the following steps: (1) preparing suspension of a tungstendisulfide nanosheet in water; (2) adding an ssDNA (single-stranded deoxyribonucleic acid) probe into the suspension of the tungsten disulfide nanosheet for uniform mixing; (3) adding a soluble metalsalt into the suspension obtained in step (2) and performing uniform mixing to obtain a first-state label-free biosensor; (4) adding a target ssDNA into the suspension obtained in step (3) and performing reaction to obtain a second-state label-free biosensor. The ssDNA probe is a complementary strand of the target ssDNA. The invention also discloses application of the label-free biosensor to detection of an ssDNA concentration. When the label-free biosensor is adopted for detection of nucleic acid, relatively low cost and simplicity for operation are achieved.

Description

technical field [0001] The invention relates to the technical field of optical biosensors, in particular to a label-free biosensor based on tungsten disulfide nanosheets and its preparation method and application. Background technique [0002] Tungsten disulfide is a layered compound with sulfur-tungsten-sulfur as the basic structure of each layer. Each layer structure is two layers of sulfur atoms sandwiching a layer of tungsten atoms, there is no dangling bond between the layers, only relying on the weak van der Waals force to combine, easy to slide along the layer. This structure allows bulk tungsten disulfide to be exfoliated to obtain two-dimensional layered tungsten disulfide nanosheets. Due to the extinction of tungsten disulfide nanosheets, there is also a Tyndall effect when dispersed in a solvent. According to its absorption of light, the ultraviolet-visible absorption spectrum of tungsten disulfide nanosheets can be seen. There are absorption peaks at 410nm and ...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 应义斌蓝玲怡平建峰
Owner ZHEJIANG UNIV
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