SSR molecular marker primer for identifying flower color traits of pink-flowered strawberries and application

A molecular marker, strawberry technology, applied in the field of molecular biology, can solve the problems of long breeding cycle, color separation of hybrid offspring, etc., and achieve the effect of shortening the breeding cycle

Active Publication Date: 2018-09-18
SHENYANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the breeding of new varieties of safflower strawberry still relies on the traditional hybrid breeding method. The breeding cycle is relatively long. It takes at least 2-3 years from hybridization to normal flowering and fruiting of seedlings, and the color of hybrid offspring is significantly separated.

Method used

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  • SSR molecular marker primer for identifying flower color traits of pink-flowered strawberries and application
  • SSR molecular marker primer for identifying flower color traits of pink-flowered strawberries and application
  • SSR molecular marker primer for identifying flower color traits of pink-flowered strawberries and application

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Embodiment Construction

[0014] Based on transcriptome sequencing, develop specific SSR molecular markers related to flower color traits of safflower strawberry. The specific methods are:

[0015] 1. Safflower strawberry transcriptome sequencing

[0016] The petals of all red-flowered seedlings (6 plants) and white-flowered seedlings (6 plants) in the full-blooming stage of the hybrid offspring of safflower strawberry pink princess × pink beauty were selected for transcriptome sequencing. Total petal RNA was extracted by CTAB method, and eukaryotic mRNA was enriched by magnetic beads with Oligo(dT). Add fragmentation buffer to break mRNA into short fragments, use the cleaved short fragments as templates, use six-base random primers (random hexamers) to synthesize the first cDNA strand, and then add buffer, dNTPs, RNase H and DNA polymerase I Synthesize the second cDNA strand, and then purify the double-stranded cDNA with AMPure XP beads. The purified double-stranded cDNA was firstly subjected to A-t...

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Abstract

The invention discloses an SSR molecular marker primer for identifying flower color traits of pink-flowered strawberries and application. Four SSR molecular marker primers, namely UgRFsr57622, UgRFsr94149, UgRFsr40142, and UgRFsr54608, are provided; the invention obtains a specific marker capable of marking red-series flowers (red, deep pink, pink and light pink) of pink-flowered strawberry filialgenerations, thereby conveniently and effectively distinguishing white flowers and other colors during the pink-flowered strawberry seedling period, and greatly shortening the breeding cycle of the pink-flowered strawberries.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a molecular marker used for identifying flower color traits of safflower strawberry. Background technique [0002] The plants of the genus Fragaria all have white flowers, and Pink-flowered strawberry (Pink-flowered strawberry) is an intergeneric hybrid (Fragaria×Potentilla), which is a new type of perennial herbaceous flower with both ornamental and edible properties (Mabberley, 2002). Safflower strawberry is deeply loved by people because of its beautiful flower color and edible fruit, and has great market potential and economic value, and the price of safflower strawberry is also much higher than that of common white-flower strawberry cultivars (Bentvelsen and Bouw, 2006). At present, the breeding of new varieties of safflower strawberry still relies on the traditional hybrid breeding method, and the breeding cycle is relatively long. It takes at least 2-3 years f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 雷家军薛莉丁言代汉萍宋雨桐郑洋毕晓颖张莹莹曲娜关婧竹赫嵩
Owner SHENYANG AGRI UNIV
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