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UPLC-MS/MS detection method of eight biogenic amines in Baijiu

A detection method, biogenic amine technology, applied in the field of liquor detection and analysis, can solve the problems of export product confiscation, economic loss, etc., and achieve the effect of simple pre-treatment, rapid and efficient detection

Active Publication Date: 2018-09-21
安徽润安信科检测科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If my country's aquatic product export enterprises do not pay attention to the detection of biogenic amines, their export products will be fined and confiscated, resulting in serious economic losses

Method used

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  • UPLC-MS/MS detection method of eight biogenic amines in Baijiu
  • UPLC-MS/MS detection method of eight biogenic amines in Baijiu
  • UPLC-MS/MS detection method of eight biogenic amines in Baijiu

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Embodiment Construction

[0056] The technical solution of the present invention will be further analyzed and illustrated through specific examples below.

[0057] 1 Instruments and samples

[0058] 1.1 Instruments:

[0059] An ultra-high performance liquid chromatography system (ACQUITY Ultra Performance LC Waters) is equipped with a mass spectrometer (MS / MS Waters), a rotary evaporator (Buchi R-210, Switzerland), and a BEH Amide (1.7 μm, 2.1×100mm) chromatographic column.

[0060] 1.2 Sample: commercial liquor

[0061] 2 Methods and results

[0062] 2.1.1 Preparation of standard products: Prepare 1g / L standard solutions of putrescine, cadaverine, histamine, tyramine, phenethylamine, spermidine, tryptamine and spermine respectively, and standard solutions of eight biogenic amines The solutions were mixed and diluted step by step to finally obtain 8 kinds of biogenic amine mixed standard solutions with concentrations of 50 μg / L, 150 μg / L, 250 μg / L, 350 μg / L, and 500 μg / L.

[0063] 2.1.2 Sample pret...

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Abstract

The invention discloses a UPLC-MS / MS detection method of eight biogenic amines in Baijiu. The eight biogenic amines such as putrescine, cadaverine, histamine, tyramine, phenylethylamine, spermidine, tryptamine and spermine in the Baijiu can be qualitatively and quantitatively analyzed at the same time. The UPLC-MS / MS detection method of the eight biogenic amines in the Baijiu, disclosed by the invention, has the advantages that pretreatment is simple and derivatization is not needed; a linear correlation coefficient is greater than 0.99, the recovery rate is 80 to 120 percent and the relativestandard deviation is within 10 percent, which shows that the method disclosed by the invention can be applied to quantitative analysis of the eight biogenic amines in the Baijiu.

Description

technical field [0001] The invention relates to a UPLC-MS / MS detection method for eight biogenic amines in liquor, belonging to the field of liquor detection and analysis. Background technique [0002] Biogenic amines are a class of nitrogen-containing aliphatic (putrescine, cadaverine, spermine, spermidine), aromatic (tyramine, phenylethylamine) or heterocyclic (histamine, tryptamine) low molecular weight compounds. It is mainly formed through the deshuttle of amino acids or the amination and transamination of aldehydes and ketones. It has important physiological effects on the active cells of animals, plants and microorganisms. An appropriate amount of biogenic amines contributes to the normal physiological functions of the human body, and excessive amounts will cause It causes adverse physiological reactions, which can lead to increased blood pressure, headache, flushing, and rash; sometimes it is mainly gastrointestinal dysfunction, including sudden vomiting and diarrhea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 刘露刘国英马雷马金同沈小梅胡心行李安军
Owner 安徽润安信科检测科技有限公司
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