HCT-8 cytopathy virus isolation culture device and method
A HCT-8, cytopathic technology, which is applied in the cultivation devices of tissue cells/viruses, viruses, viruses/phages, etc., can solve the problems of easy mixed occurrence and increased mortality, and achieve the effect of improving flexibility
Inactive Publication Date: 2018-09-25
JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE
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Problems solved by technology
Bovine coronavirus and rotavirus infection are similar, and the two are prone to mix, resulting in increased mortality
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[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0020] see Figure 1-2 , the present invention provides a technical solution: a device and method for separating and culturing HCT-8 cytopathic virus, comprising a carbon dioxide incubator 1, a box top 2, a display screen 3, an adjustment knob 4, a timer 5, a test tube box 6, and a base 7. Box door 8, post-it note 9, small blackboard 10, observation window 11, workbench 12, box cover 13, hinge 14, cushion 15, test tube rack 16, connecting seam 17 and hinge 18,...
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The invention discloses an HCT-8 cytopathy virus isolation culture device and method. The device comprises a carbon dioxide culture tank and a base and is characterized a tank top is mounted at the top of the carbon dioxide culture tank, adjusting knobs are arranged on the right of a display screen, a tank door located below the tank top is mounted on the carbon dioxide culture tank, a post-it note and a small blackboard are mounted on the tank door below an observation window, a workbench is arranged on the right side of the top of the base, a test tube tank is arranged on the left side of the top of the base, the middle of the left side of the test tube tank is provided with a connecting joint, hinges are mounted at the connecting joint, a cushion layer is arranged at the bottom of the inner side of the test tube tank, and a test tube rack is arranged at the top of the cushion layer. The device has the advantages that the carbon dioxide culture tank and the test tube tank are combined, the carbon dioxide culture tank, the test tube tank and the workbench are used in a coordinated manner, and use flexibility is increased; in addition, a timer is added, and convenience is brought to experiment operation by using the timer, the post-it note and the small blackboard.
Description
technical field [0001] The invention relates to the technical field of virus isolation and culture, in particular to a device and method for the isolation and culture of HCT-8 cytopathic virus. Background technique [0002] Bovine coronavirus is the main pathogen that causes adult bovine winter scour, neonatal calf diarrhea and respiratory disease. Adult cattle have severe watery diarrhea in winter, with high morbidity and low mortality, while calves infection is more common within one week of age, and diarrhea often occurs at 7-10 days of age, with watery diarrhea as the main manifestation, seriously affecting the growth of calves performance. Bovine coronaviruses are similar to rotavirus infections, and the two are prone to co-occurrence, resulting in increased mortality. Animals infected with coronavirus can excrete toxins to the environment through feces, and even 18 months after symptoms recover, they can still excrete invasive viruses, which has a serious impact on t...
Claims
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IPC IPC(8): C12M1/34C12M3/00C12M1/00C12N7/00
CPCC12M41/00C12M23/00C12N7/00C12N2770/20051
Inventor 贺生中管远红李芙蓉孟婷谢静傅宏庆程汉陈长春蔡丙严邱世华
Owner JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE