Preparation method and application of brown algae polyphenol

A technology of brown algae polyphenols and brown algae, which is applied in the field of preparation of brown algae polyphenols, can solve the problems of low extraction efficiency and low utilization rate of raw materials, and achieve the effects of high extraction efficiency, high utilization rate of raw materials, and remarkable therapeutic and preventive effects

Inactive Publication Date: 2018-10-09
深圳市盛东华科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the extraction efficiency of brown algae polyphenols from brown algae is low, the utilization rate of ...

Method used

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  • Preparation method and application of brown algae polyphenol
  • Preparation method and application of brown algae polyphenol
  • Preparation method and application of brown algae polyphenol

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[0018] The invention provides a method for preparing brown algae polyphenols, comprising the steps of:

[0019] Freeze the brown algae at a temperature of -30 to -40°C, take it out after freezing for 15 to 20 days, and thaw at room temperature until the temperature of the brown algae reaches 0 to 1°C;

[0020] Put the thawed brown algae in a centrifuge to remove water;

[0021] adding an organic solvent to the brown algae after water removal, extracting in the dark for 1 to 12 hours, and then suction filtering to obtain an extract;

[0022] Concentrate the extract at 30-40°C until the amount of precipitate in the solution no longer increases, and filter to remove the precipitate to obtain the first filtrate;

[0023] Crystallize the first filtrate at a low temperature of 5-20°C for 5-10 hours, and filter to obtain the second filtrate;

[0024] adding a fat-soluble solvent to the second filtrate, performing liquid-liquid extraction, and retaining a fat-soluble solvent layer; ...

Embodiment 1

[0036] Freeze the fresh wakame at -40°C, take it out after 15 days of freezing, and thaw it in air at room temperature until the center temperature of the wakame reaches 1°C to complete the freeze-thaw process; put the wakame into a centrifuge , centrifuged at 2000rpm for 10min to remove moisture; through repeated freeze-thaw and centrifugation for 3 times, the water content of Undaria pinnatifida was controlled to be 60%. Weigh 100g of Undaria pinnatifida and put it into a 1000mL conical beaker, add 300mL of 85% (V / V) ethanol aqueous solution, seal the mouth of the conical beaker with plastic wrap, leaching in the dark for 1 hour, and suction filtration. Continue to add 85% (v / v) ethanol aqueous solution 300mL again to the above-mentioned leaching treatment of wakame, seal the mouth of the triangular beaker with a safety film, leaching in the dark for 1 hour, and suction filtration. Repeatedly leached in the dark until the extract was colorless, and the filtrates were combine...

Embodiment 2

[0039] Freeze the fresh hijiki at -30°C, take it out after 20 days of freezing, and thaw it in the air at room temperature until the center temperature of the hijiki reaches 1°C. put into a centrifuge, and centrifuge at a speed of 1200rpm for 20min to remove water; through repeated freezing and thawing and centrifugation twice, the moisture content of Hijiki is controlled to be 60%. Weigh 100g of Hijiki and put it into a 1000mL conical beaker, add 300mL of 80% (V / V) ethanol aqueous solution, seal the mouth of the conical beaker with plastic wrap, extract in the dark for 1 hour, and filter with suction. Continue to add 300 mL of 80% (v / v) ethanol aqueous solution to the above-mentioned leaching treatment of Hijiki again, seal the mouth of the triangular beaker with a safety film, leaching in the dark for 1 hour, and suction filtration. Repeatedly leached in the dark until the extract was colorless, and the filtrates were combined. Concentrate the filtrate at 40°C. During conce...

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Abstract

The invention provides a preparation method and application of brown algae polyphenol. The preparation method comprises the following steps: freezing brown algae at the temperature of -30 to -40 DEG Cfor 15 to 20 days, and thawing the brown algae at room temperature until the brown algae is at 0 to 1 DEG C; centrifuging the thawed brown algae in a centrifugal machine to remove water; adding an organic solvent into the brown algae without water, carrying out extraction for 1 to 12 hours in the dark, and then carrying out suction filtration to obtain an extract solution; concentrating the extract solution at 30 to 40 DEG C until the amount of precipitates in the solution is no longer increased, and filtering to remove the precipitates, so as to obtain a first filtrate for standby use; crystallizing the first filtrate at a low temperature of 5 to 20 DEG C for 5 to 10 hours, and filtering to obtain a second filtrate; adding a fat-soluble solvent into the second filtrate, and performing liquid-liquid extraction to obtain a fat-soluble solvent layer; and concentrating the fat-soluble solvent layer at 20 to 30 DEG C until a yellow-green solid is separated out of the solution, so as to obtain the brown algae polyphenol.

Description

technical field [0001] The invention relates to the field of natural material preparation, in particular to a preparation method and application of brown algae polyphenols. Background technique [0002] Osteoporosis (osteoporosis) is a chronic bone disease characterized by bone tissue degeneration, osteopenia and susceptibility to fracture. The imbalance of osteoclast and osteoblast function is the direct cause of this pathological process. The main manifestations are It is chronic low back pain, low back deformities and fractures, and middle-aged and elderly people are prone to suffer from it. With the aging of human society, the incidence of osteoporosis continues to increase. Statistics show that about 200 million women worldwide suffer from osteoporosis, especially among the elderly. About 1 / 3 of women between the ages of 60 and 70 suffer from osteoporosis; women aged 80 or above , about 2 / 3 suffer from osteoporosis, in China, the total number of osteoporosis patients h...

Claims

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Application Information

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IPC IPC(8): A61K36/03A61P19/10
CPCA61K36/03A23V2002/00A61K2236/333A61K2236/51A61K2236/53A23V2200/306A23V2250/202A23V2250/2132
Inventor 曾小斌王雪艳吴晓娜黄泳怡唐旭东
Owner 深圳市盛东华科技有限公司
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