Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Synthesis method of EPS-RB nanometer particles for photodynamic bacterium resistance

An EPS-RB, photodynamic antibacterial technology, applied in the field of biotechnology and nanomaterials, can solve the problems of high cytotoxicity, poor antibacterial effect, and poor biological safety, and achieve low cytotoxicity, good photodynamic antibacterial effect, and small size uniform effect

Active Publication Date: 2018-10-09
SOUTHEAST UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for improved killing efficiency against harmful microorganisms by producing small particles called epsilium (EPO) or silicate glasses with specific sizes and properties. These tiny objects can be used alone without affecting their functioning effectively at high dosages but they also help fight off other germs like viruses.

Problems solved by technology

Technologies described in this patent involve developing new ways to chemically modify certain compounds called photoinitiators without affecting their function when exposed outside them. One approach involves combining different types of organics together like photosensitisiders and amino acids onto small particles made of silicon diozone or quantum dots instead of bulky metals. Additionally, adding another type of material between two layers helps reduce interference during imaging processes due to sterilization effects. Overall, these techniques aim to create safe and efficient pharmaceutical compositions containing antihyperlipidant(AHL)-mediator complexes.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Synthesis method of EPS-RB nanometer particles for photodynamic bacterium resistance
  • Synthesis method of EPS-RB nanometer particles for photodynamic bacterium resistance
  • Synthesis method of EPS-RB nanometer particles for photodynamic bacterium resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Lactobacillus plantarum exopolysaccharide EPS and photosensitizer Rose Bengal (RB) synthesize EPS-RB nanoparticles:

[0037]Make a 1mg / mL solution of exopolysaccharide and let it stand overnight; weigh 2-5mg RB, 5-12mg EDC·HCl and 2-64.8mg NHS, dissolve in 9mL DMSO, and react at room temperature and 90rpm for 1-3h Finally, it was mixed with the prepared polysaccharide solution, reacted overnight at room temperature and 90 rpm, then dialyzed with DMSO for 72 hours, then dialyzed with water for 48 hours, and stored in freeze-dry.

Embodiment 2

[0038] The morphology observation of the synthesized EPS-RB nanoparticle of embodiment 2:

[0039] 10 μL of the EPS-RB nanoparticles obtained in Example 1 above was dropped onto a 400-mesh copper grid for transmission electron microscope observation. EPS-RB nanoparticles have a spherical structure and are evenly distributed ( figure 1 ). The particle size distribution statistics show that the nanoparticle size is about 35.2nm ( figure 2 ).

Embodiment 3

[0040] Embodiment 3EPS-RB nanoparticle stability evaluation:

[0041] Stability evaluation is carried out by measuring particle size and zeta potential. The EPS-RB nanoparticles were dissolved in 0.9% NaCl to form a 10uM solution, and then the particle size was measured by DLS, and the charge was measured by a zeta potentiometer.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a synthesis method of EPS-RB nanometer particles for photodynamic bacterium resistance. Lactobacillus plantarum exopolysaccharides EPS, rose-bengal RB, 1-(3-dimethylaminopropyl)-3-ethylenediamine hydrochloride EDC-HCl and N-hydroxysuccinimide NHS are used as raw materials; reaction is performed in dimethyl sulfoxide DMSO to obtain compounds EPS-RB; the compounds EPS-RB aresubjected to self assembly to form spherical nanometer particles in a water solution; a good photodynamic bacterium resistance effect is achieved. The compound can realize the self aggregation in thesolution to form nanometer particles; the nanometer particles generates singlet oxygen higher than free RB under the light illumination condition, so that the photodynamic bacterium resistance on escherichia coli and staphylococcus aureus is superior to that of the free RB. The concentration required by EPS-RB nanometer particles for completely killing escherichia coli and staphylococcus aureus isrespectively 8mu M and 500nM; the concentration required by the free RB is respectively 16 mu M and 1000nM.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com