PCR (polymerase chain reaction) Primer pair and method to detect Schlumbergera virus X and application of PCR primer pair

A technology of Pylocagna and primer pairs, which is applied in the field of PCR primer pairs used in the detection of Pyreum chinensis X virus, can solve the problems of dragon fruit yield decline, poor quality, lack of treatment measures, etc., and achieve high detection sensitivity and simple operation Effect

Pending Publication Date: 2018-10-09
GUIZHOU FRUIT INST
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Problems solved by technology

Among the viral diseases infecting dragon fruit, Crabella X virus is a relatively common one on dragon fruit, which leads to a decrease in the yield and quality of dragon fruit
However, for this disease, there is still a lack of effective treatment measures in China. To control the spread of the disease, the first task is to ensure that the seedlings are non-toxic and safe. As for the virus, there are few domestic reports and a complete detection system has not been established.

Method used

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  • PCR (polymerase chain reaction) Primer pair and method to detect Schlumbergera virus X and application of PCR primer pair
  • PCR (polymerase chain reaction) Primer pair and method to detect Schlumbergera virus X and application of PCR primer pair
  • PCR (polymerase chain reaction) Primer pair and method to detect Schlumbergera virus X and application of PCR primer pair

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Embodiment Construction

[0028] The specific embodiments provided by the present invention will be described in detail below in conjunction with the accompanying drawings, and the advantages and characteristics of the present invention will become clearer along with the description.

[0029] refer to Figure 1-Figure 3 : The detailed technical scheme that the present invention takes is as follows: detect the used PCR primer pair of Crabella X virus, comprise upstream primer SchVX CP-F: 5 '-GGTCATCACCGGAAACTGT-3 ', downstream primer SchVX CP-R: 5 '-CTTTGGGCTTTCAGATCCTT-3 '. The PCR primer pair can be applied in detecting whether the pitaya, cactus, and crab claws are infected by the crab claws X virus.

[0030] The specific detection method steps are as follows:

[0031] 1. Extract the total RNA of the dragon fruit sample to be tested, using the Plant Column RNA Extraction Kit from Bao Bioengineering (Dalian) Co., Ltd. (Cat. No. 9769), and operate according to the method provided by the kit to extrac...

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Abstract

The invention discloses a PCR (polymerase chain reaction) primer pair and method to detect Schlumbergera virus X and application of the PCR primer pair. The primer pair includes an upstream primer SchVX CP-F: 5'-GGTCATCACCGGAAACTGT-3', and a downstream primer SchVX CP-R: 5'-CTTTGGGCTTTCAGATCCTT-3'. The PCR primer pair can be applied to the detection on whether Hylocereus undulatus, Opuntia strictaand Schlumbergera truncata experience Schlumbergera virus X infection.

Description

technical field [0001] The invention relates to the technical field of molecular biology and plant virus detection, in particular to a pair of PCR primers used for detection of Ceratum X virus, a detection method and an application thereof. technical background [0002] Dragon fruit (Hylocereus undatus Britt.), as a typical tropical fruit plant, belongs to the genus Hylocereus of the family Cactaceae, and has developed rapidly in the southern provinces of my country in recent years. In the late 1990s, the mainland began to introduce dragon fruit planting. By 2017, my country's dragon fruit planting area was about 550,000 mu, of which Guizhou had a planting area of ​​130,200 mu, making it the largest planting province in China. Since the propagation method of dragon fruit is mainly cutting propagation, the spread of viral diseases can be accelerated. As the area with the largest dragon fruit planting area in China, Guizhou has found in recent years that the symptoms of viral ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11C12N15/70C12R1/94
CPCC12N15/70C12Q1/686C12Q1/701C12Q2565/125
Inventor 柏自琴郑乾明李兴忠王小柯刘涛马玉华
Owner GUIZHOU FRUIT INST
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