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Mulberry polyploid induction method based on tissue culture seedling

A technology for polyploid and tissue culture seedlings, which can be applied in horticultural methods, botanical equipment and methods, plant genetic improvement and other directions, can solve problems such as low chemical mutagenesis rate of mulberry, and achieve the effect of improving chemical mutagenesis rate.

Inactive Publication Date: 2018-10-12
NORTHWEST A & F UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, the embodiment of the present invention provides a method for inducing mulberry polyploids based on tissue culture seedlings, the main purpose of which is to solve the problem of low chemical mutagenesis rate of mulberry

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  • Mulberry polyploid induction method based on tissue culture seedling
  • Mulberry polyploid induction method based on tissue culture seedling
  • Mulberry polyploid induction method based on tissue culture seedling

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Embodiment

[0028] (1) Get aseptic mulberry tissue culture seedling as polyploid induction material;

[0029] (2) Induction of mulberry polyploid: Cut the mulberry tissue culture seedlings into 6-8cm stem segments in the ultra-clean bench, remove the leaves and leave a little petiole, transfer to the pre-configured and sterilized culture medium A, Then seal the culture dish containing the culture solution A with a parafilm, put it in a low-speed shaker at 250-1000r / min, and induce culture for 48 hours by shaking in the dark, at a culture temperature of 25±1°C; the formula of the culture solution A above is MS+ 0.1% colchicine liquid medium;

[0030] (3) Post-induction cultivation: Take out the above-mentioned stem section cultured by dark shaking in the ultra-clean bench, cut off the stem section with both ends contacting colchicine on the sterilized petri dish, and then cut it into 1-2 stem sections. For the small stem section of the bud, the rear end of the bud is slightly longer when ...

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Abstract

The invention discloses a mulberry polyploid induction method based on a tissue culture seedling and relates to the technical field of agricultural breeding. The mulberry tissue culture seedling is used as a polyploid to-be-induced material; the to-be-induced material is prepared into a first stem segment with the length of 6-8 cm; the first stem segment is subjected to oscillation induction culture in a culture solution A to obtain a first induction material; the two ends,in contact with the culture solution A,of the stem segment are removed to obtain a second induction material; the second induction material is prepared into multiple third stem segments with 1-2 buds; the third stem segments are transferred to a solid culture medium B for culturing to obtain a polyploid material; the polyploid material is subjected to ploidy identification,and a polyploid pure material is screened out. By using the chemical induction method for performing chemical induction on the mulberry polyploidtissue culture seedling,the chemical mutagenicity rate of mulberries is increased,more mutagenic breeding materials are quickly obtained,and new materials are provided for breeding mulberry varieties.

Description

technical field [0001] The invention relates to the technical field of agricultural breeding, in particular to a method for inducing mulberry polyploids based on tissue culture seedlings. Background technique [0002] Tissue culture seedlings are based on the theory that plant cells are totipotent, using isolated plant organs (such as roots, stems, leaves, shoot tips, flowers, fruits, etc.), tissues (such as cambium, epidermis, cortex, pith cells, etc.) , endosperm, etc.), cells (such as megaspores, microspores, somatic cells, etc.) and protoplasts can induce callus, adventitious buds, Adventitious roots eventually form complete plants. [0003] Mulberry is an important perennial woody economic plant and an important material basis for the development of silk industry and ecological mulberry industry. Because its biological yield mainly comes from vegetative bodies, polyploidy mutagenesis breeding plays an important role in mulberry breeding. Compared with diploid, polypl...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/001A01H4/008
Inventor 包立军苏超焦锋张敏娟赵晓晓梁嘉俊钱永华
Owner NORTHWEST A & F UNIV
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