Multidimensional complex enzyme inoculant as well as preparation method and applications of multidimensional complex enzyme inoculant

A compound enzyme agent and multi-dimensional technology, applied in the biological field, achieves the effects of strong enzyme production ability, scientific strain matching, and unique fermentation method

Inactive Publication Date: 2018-10-12
祺兴生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the bacteria or enzyme preparations currently used as feed or feed additives still need to be improved in terms of improving animal absorption efficiency, feed utilization efficiency, and intestinal peristalsis. Therefore, new products need to be developed

Method used

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  • Multidimensional complex enzyme inoculant as well as preparation method and applications of multidimensional complex enzyme inoculant

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preparation example Construction

[0022] The present invention provides a kind of preparation method of multi-dimensional composite enzyme bacterial agent, comprising steps:

[0023] S1: (1) Preparation of bacterial mixed fermentation broth

[0024] Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus jelly-like and Bacillus licheniformis were respectively cultured and activated on a slant at 37°C for 2-4 hours, and then a nutrient broth medium was used for an expansion culture. : The temperature is 37°C, the rotating speed of the shaker is 200r / min, and the culture time is 12h; wherein, the components of each L of nutrient broth medium include: peptone 10g, beef extract powder 3g, sodium chloride 5g, and the balance is water, pH 7.4;

[0025]Inoculate the once-expanded Bacillus subtilis, the once-expanded Bacillus amyloliquefaciens, the once-expanded jelly-like Bacillus and the once-expanded Bacillus licheniformis into standard seed tanks according to the inoculation amount of 3% to 5%. (200L), carry out...

Embodiment 1

[0041] The present embodiment provides a kind of preparation method of multi-dimensional compound enzyme bacterial agent, comprises steps:

[0042] S1: (1) Preparation of bacterial mixed fermentation broth

[0043] Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus jelly-like and Bacillus licheniformis were cultured and activated on a slant surface at 37°C for 3 hours, and then expanded culture was carried out with nutrient broth medium respectively. During the expanded culture process: the temperature was 37°C, the rotation speed of the shaker is 200r / min, and the culture time is 12h; the components of each L of nutrient broth medium include: peptone 10g, beef extract powder 3g, sodium chloride 5g, the balance is water, pH The value is 7.4;

[0044] Inoculate the Bacillus subtilis once expanded, the Bacillus amyloliquefaciens amyloliquefaciens once expanded, the bacillus jelly-like bacillus and the Bacillus licheniformis once expanded culture according to the inoculatio...

Embodiment 2

[0059] The present embodiment provides a kind of preparation method of multi-dimensional compound enzyme bacterial agent, comprises steps:

[0060] S1: (1) Preparation of bacterial mixed fermentation broth

[0061] Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus jelly-like and Bacillus licheniformis were respectively cultured and activated on a slant at 37°C for 2 hours, and then the nutrient broth medium was used to expand the culture once. During the expansion culture process: the temperature was 37°C, the rotation speed of the shaker is 200r / min, and the culture time is 12h; the components of each L of nutrient broth medium include: peptone 10g, beef extract powder 3g, sodium chloride 5g, the balance is water, pH The value is 7.4;

[0062] Inoculate the once-expanded Bacillus subtilis, the once-expanded Bacillus amyloliquefaciens, the once-expanded jelly-like Bacillus and the once-expanded Bacillus licheniformis into standard seed tanks according to the inoculation...

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Abstract

The invention relates to a multidimensional complex enzyme inoculant as well as a preparation method and applications of the multidimensional complex enzyme inoculant. The preparation method comprisesthe following steps: mixing bacterial mixed fermentation liquor, a mould mixture and a saccharomycete mixture, thus obtaining a strain mixture; inoculating the strain mixture into a solid fermentation culture medium, thus obtaining a fermentation mixture; adjusting the moisture of the fermentation mixture, carrying out stacking to form a conical pile, and carrying out fermentation, thus obtaininga primary fermentation product; carrying out anaerobic fermentation on the primary fermentation product, thus obtaining a secondary fermentation product; and drying the secondary fermentation product, and carrying out grinding, thus obtaining the multidimensional complex enzyme inoculant. According to the technical scheme, various strains including bacillus subtilis, bacillus amyloliquefaciens,bacillus mucilaginosus, bacillus licheniformis, trichoderma harzianum, aspergillus oryzae, pichia farinose and an aroma-producing yeast are adopted as the fermentation strains, then the multidimensional complex enzyme inoculant containing various efficient enzymes, beneficial substances and beneficial microbial populations and having the high-activity fermentation function is prepared, and the multidimensional complex enzyme inoculant can be used for preparing organic fertilizers, feeds and feed additives.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a multi-dimensional compound enzyme bacterial agent and its preparation method and application. Background technique [0002] With the improvement of people's living standards, people pay more and more attention to the safety of food. However, in our country, the abuse of antibiotics and other antibacterial drugs has caused drug residues and a large number of drug-resistant bacteria. As a result, the healthy and sustainable development of the livestock and poultry industry has been seriously hindered, and hidden dangers have been laid for food safety and the health of consumers. Therefore, it is urgent to find safe and residue-free alternatives to antibiotics. Biological feed additives containing probiotics and enzymes are ideal alternatives to antibiotics. Among them, enzyme is a special protein with the ability to catalyze the reaction, and it is an efficient substance to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00A23K20/189C05F11/08
CPCA23K20/189C05F11/08C12N9/00
Inventor 王祺中孙桂彩
Owner 祺兴生物技术有限公司
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