Nucleic acid extraction and PCR (polymerase chain reaction) amplification test integrated solution

A nucleic acid and extraction unit technology, applied in the field of integrated solutions, can solve the problems of easy dripping of magnetic sleeves in instruments and reaction cups, large eluent volume requirements, unfavorable concentration of nuclear extraction samples, etc.

Active Publication Date: 2018-10-16
JIAXING ACCUNOME BIOTECHNOLOGY CO LTD
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Problems solved by technology

This method also realizes the automation of nucleic acid extraction, but the magnetic beads and liquid adsorbed on the magnetic sleeve are easy to drip into the instrument and cuvette during the transfer process, which will cause system po

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  • Nucleic acid extraction and PCR (polymerase chain reaction) amplification test integrated solution
  • Nucleic acid extraction and PCR (polymerase chain reaction) amplification test integrated solution
  • Nucleic acid extraction and PCR (polymerase chain reaction) amplification test integrated solution

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[0049] The present invention will be further elaborated below in conjunction with the accompanying drawings and specific embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the protection scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also belong to the scope defined by the appended claims of the present application.

[0050] Such as figure 1 and image 3 As shown, the embodiment of the present invention consists of a sample pretreatment unit 1 , a liquid transfer unit 2 , a nucleic acid extraction unit 3 , a PCR amplification detection unit 4 and an anti-pollution unit 5 . The above-mentioned units are integrated into one kit, which is the integrated detection kit of this embodiment.

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Abstract

The invention discloses a nucleic acid extraction and PCR (polymerase chain reaction) amplification test integrated kit and a test method thereof. The kit comprises a nucleic acid extraction unit anda PCR amplification test unit, and can cooperate with an assorted instrument to complete a full-automatic process of nucleic acid extraction and PCR amplification test; in the process, no transferringoperation or manual intervention is needed. The nucleic acid extraction and PCR amplification test integrated solution realizes real automation, reduces the instrument cost, shortens the time of thewhole process, avoids the pollution in a nucleic acid extraction process, improves the purity of nucleic acid extraction and accelerates the PCR amplification process, so that the reliability of the experiment and the experimental efficiency are improved.

Description

technical field [0001] The invention relates to an experimental method for molecular biology analysis, in particular to an integrated solution combining nucleic acid extraction and PCR amplification detection. Background technique [0002] Nucleic acid extraction and PCR amplification detection are important experimental methods in molecular biology research. The early experiments were all done by hand, which took a long time and the experimental results were easily affected by the differences in individual experimenters' operations. With the development of industrial automation, in the field of experimental science, traditional manual experiments are increasingly replaced by automated equipment. The repeatability and consistency of automated equipment reduces the error of traditional manual experiments and improves the reliability of experiments. performance and experimental efficiency. [0003] [Experimental procedures] In terms of experimental procedures, the general nuc...

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Application Information

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IPC IPC(8): C12M1/38C12M1/34C12M1/12C12M1/00C12Q1/6806C12Q1/686
CPCC12Q1/6806C12Q1/686C12Q2563/107
Inventor 王宪华徐辉张伟宋扬
Owner JIAXING ACCUNOME BIOTECHNOLOGY CO LTD
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