Nucleic acid extraction and PCR (polymerase chain reaction) amplification test integrated solution

Abstract

The invention discloses a nucleic acid extraction and PCR (polymerase chain reaction) amplification test integrated kit and a test method thereof. The kit comprises a nucleic acid extraction unit anda PCR amplification test unit, and can cooperate with an assorted instrument to complete a full-automatic process of nucleic acid extraction and PCR amplification test; in the process, no transferringoperation or manual intervention is needed. The nucleic acid extraction and PCR amplification test integrated solution realizes real automation, reduces the instrument cost, shortens the time of thewhole process, avoids the pollution in a nucleic acid extraction process, improves the purity of nucleic acid extraction and accelerates the PCR amplification process, so that the reliability of the experiment and the experimental efficiency are improved.

Description

technical field [0001] The invention relates to an experimental method for molecular biology analysis, in particular to an integrated solution combining nucleic acid extraction and PCR amplification detection. Background technique [0002] Nucleic acid extraction and PCR amplification detection are important experimental methods in molecular biology research. The early experiments were all done by hand, which took a long time and the experimental results were easily affected by the differences in individual experimenters' operations. With the development of industrial automation, in the field of experimental science, traditional manual experiments are increasingly replaced by automated equipment. The repeatability and consistency of automated equipment reduces the error of traditional manual experiments and improves the reliability of experiments. performance and experimental efficiency. [0003] [Experimental procedures] In terms of experimental procedures, the general nuc...

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Problems solved by technology

This method also realizes the automation of nucleic acid extraction, but the magnetic beads and liquid adsorbed on the magnetic sleeve are easy to drip into the instrument and cuvette during the transfer process, which will cause system pollution

In addition, the volume of eluent required by this method is large, which is not conducive to the concentration of nuclear extraction samples, and the demand for high concentration of nuclear extraction samples is not easy to achieve

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