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Fluorescence determination method based on nucleic acid aptamer-quantum dot in combination with bacillus thuringiensis spore

A nucleic acid aptamer and Bacillus aureus technology, which is applied in the field of fluorescence assay, can solve problems such as unsatisfactory, and achieve the effect of easy determination and short detection cycle

Inactive Publication Date: 2018-10-16
武汉世格美检测技术有限公司
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Problems solved by technology

[0006] However, as the requirements for detection sensitivity are getting higher and higher, the detection of genetically modified components and the detection of proteins at two levels can no longer meet the needs of current development.

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  • Fluorescence determination method based on nucleic acid aptamer-quantum dot in combination with bacillus thuringiensis spore
  • Fluorescence determination method based on nucleic acid aptamer-quantum dot in combination with bacillus thuringiensis spore
  • Fluorescence determination method based on nucleic acid aptamer-quantum dot in combination with bacillus thuringiensis spore

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Embodiment Construction

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0034] 1. Preparation of Cry1Ab protein

[0035] The preparation steps of Cry1Ab protein are as follows: see Figures 1 to 2 As shown, the Bacillus thuringiensis strains stored in the -70°C refrigerator were streaked onto LB plates and grown overnight at 30°C, and a single colony was picked and cultured in 10 mL of LB based on shaking at 30°C overnight. Then add the overnight cultured bacterial solution into 200 mL of PGSM medium and shake and culture at 30° ...

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Abstract

The invention discloses a fluorescence determination method based on nucleic acid aptamer-quantum dot in combination with bacillus thuringiensis spore. The method comprises the following steps of preparing a nucleic acid aptamer-quantum dot mixture of Cry1Ab protein namely Bt protein, and combining nucleic acid aptamer-quantum dot with bacillus spore. The method has the advantages that a Bt protein result in transgenic rice is detected through a method of combination of nucleic acid aptamer-quantum dot and bacillus spore, the detection result is objective and easy to judge, contents of transgenic products can be quantitatively analyzed, meanwhile the detection speed is high, and the detection sensitivity is high.

Description

technical field [0001] The invention belongs to the technical field of detection of transgenic components, and in particular relates to a fluorescence measurement method based on nucleic acid aptamer-quantum dots combined with Bacillus thuringiensis spores. Background technique [0002] The safety assessment of GMOs is the prerequisite for the marketization and commercialization of GMOs and their products, and the detection of GMO ingredients is an important content in the safety assessment. [0003] my country's research on genetically modified rice is at the leading level in the world. Among them, some disease-resistant and insect-resistant genetically modified rice strains have applied for biosafety certificates for production and planting, but due to some reasons such as their genetically modified organism safety issues, no strains have been approved so far. commercial production applications. In view of the maturity of transgenic rice technology and the marketization tr...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 刘军安
Owner 武汉世格美检测技术有限公司
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